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Product Info Summary
| SKU: | M01280-2 |
|---|---|
| Size: | 200ug |
| Reactive Species: | Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Plant, Rabbit, Rat, Sheep, Xenopus, Silkworm |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-HSP60 HSPD1 Monoclonal Antibody
SKU/Catalog Number
M01280-2
Size
200ug
Form
liquid
Description
Boster Bio Anti-HSP60 HSPD1 Monoclonal Antibody catalog # M01280-2. Tested in ELISA, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP60 HSPD1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01280-2)
Host
Mouse
Contents
PBS, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
LK1
Isotype
IgG1
Immunogen
Recombinant human HSP60
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~60kDa.
Reactive Species
M01280-2 is reactive to HSP60 in Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Plant, Rabbit, Rat, Sheep, Xenopus, Silkworm
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
20697 MW
Background of HSP60
In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01280-2 is guaranteed for ELISA, Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1, (M01280-2) . Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20°C . Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (M01280-2) at 1:1000 for senescence. Courtesy of: Valentina di Felice, University of Palermo, Italy.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (M01280-2) . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20°C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (M01280-2) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
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Figure 2. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-2).
HSPD1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPD1 Antibody (M01280-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
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Figure 3. Western blot analysis of HSPD1 using anti-HSPD1 antibody (M01280-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPD1 antigen affinity purified polyclonal antibody (Catalog # M01280-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSPD1.
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Figure 4. IHC analysis of HSPD1 using anti-HSPD1 antibody (M01280-2).
HSPD1 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPD1 Antibody (M01280-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HSP60 (Source: Uniprot.org, NCBI)
Gene Name
HSP60
Full Name
60 kDa heat shock protein, mitochondrial
Weight
20697 MW
Superfamily
chaperonin (HSP60) family
Alternative Names
CPN60, GROEL, HLD4, HSP 60, HSP65, HSPD1, HuCHA60, SPG 13 HSPD1 CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13 heat shock protein family D (Hsp60) member 1 60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|P60 lymphocyte protein|chaperonin 60|epididymis secretory sperm binding protein|heat shock 60kDa protein 1 (chaperonin)|heat shock protein 65|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSP60, check out the HSP60 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSP60: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP60 HSPD1 Monoclonal Antibody (M01280-2)
Hello CJ!
M01280-2 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats
Hepatic mitochondrial and ER stress induced by defective PPAR? signaling in the pathogenesis of hepatic steatosis
Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...
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