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Product Info Summary
| SKU: | M03050 |
|---|---|
| Size: | 50μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HSP47 SERPINH1 Antibody (Monoclonal, M16.10A1)
SKU/Catalog Number
M03050
Size
50μg/vial
Form
Liquid
Description
Boster Bio Anti-HSP47 SERPINH1 Antibody (Monoclonal, M16.10A1) catalog # M03050. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP47 SERPINH1 Antibody (Monoclonal, M16.10A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03050)
Host
Mouse
Contents
Each vial contains 50% glycerol and 0.09% sodium azide.
Clonality
Monoclonal
Clone Number
M16.10A1
Isotype
Mouse IgG
Immunogen
Native rat HSP47.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M03050 is reactive to SERPINH1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
46441 MW
Background of Hsp47
Heat shock protein 47, also known as SERPINH1 or HSP47, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. It has been found that HSP47 monitors the integrity of the triple helix of type I procollagen at the ER/cis-Golgi boundary and, when absent, the rate of transit from the ER to the Golgi is increased and the helical structure is compromised.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03050 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1, 000, ECL, Human, Mouse, Rat
IHC, 1:50, Human, Mouse, Rat
Validation Images & Assay Conditions
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Western blot analysis of HSP47 expression in recombinant rat HSP47 protein (lane 1), Rat-2 whole cell lysates (heat shocked) (lane 2), L929 whole cell lysates (heat shocked) (lane 3), 3T3 whole cell lysates (heat shocked) (lane 4), CHO whole cell lysates (heat shocked) (lane 5) and HELA whole cell lysates (heat shocked) (lane 6). HSP47 at 47KD was detected using mouse anti-HSP47 Antigen Affinity purified monoclonal antibody (Catalog # M03050) at 1:1000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1001).
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HSP47 was detected in paraffin-embedded sections of rat carotid artery tissue using mouse anti-HSP47 Antigen Affinity purified monoclonal antibody (Catalog # M03050) at 1:50. The immunohistochemical section was developed using SABC method (Catalog # SA1021).
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HSP47 was detected in paraffin-embedded sections of human breast cancer tissue using mouse anti-HSP47 Antigen Affinity purified monoclonal antibody (Catalog # M03050) at 1:50. The immunohistochemical section was developed using SABC method (Catalog # SA1021).
Protein Target Info & Infographic
Gene/Protein Information For SERPINH1 (Source: Uniprot.org, NCBI)
Gene Name
SERPINH1
Full Name
Serpin H1
Weight
46441 MW
Superfamily
serpin family
Alternative Names
Serpin H1;47 kDa heat shock protein;Arsenic-transactivated protein 3;AsTP3;Cell proliferation-inducing gene 14 protein;Collagen-binding protein;Colligin;Rheumatoid arthritis-related antigen RA-A47;SERPINH1;CBP1, CBP2, HSP47, SERPINH2;PIG14; SERPINH1 AsTP3, CBP1, CBP2, HSP47, OI10, PIG14, PPROM, RA-A47, SERPINH2, gp46 serpin family H member 1 serpin H1|47 kDa heat shock protein|arsenic-transactivated protein 3|cell proliferation-inducing gene 14 protein|collagen binding protein 1|colligin-1|colligin-2|rheumatoid arthritis A-47|rheumatoid arthritis-related RA-A47|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)|serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2)|serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINH1, check out the SERPINH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP47 SERPINH1 Antibody (Monoclonal, M16.10A1) (M03050)
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