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Product Info Summary
| SKU: | PB9634 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HSF4 Antibody Picoband®
SKU/Catalog Number
PB9634
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSF4 Antibody Picoband® catalog # PB9634. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9634)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HSF4, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9634 is reactive to HSF4 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
53011 MW
Background of HSF4
Heat shock factor protein 4 is a protein that in humans is encoded by the HSF4 gene. It is mapped to 16q21. Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9634 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, MCF-7 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HSF4 using anti-HSF4 antibody (PB9634).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug,
Lane 2: MCF-7 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSF4 antigen affinity purified polyclonal antibody (Catalog # PB9634) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSF4 at approximately 53 kDa. The expected band size for HSF4 is at 53 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HSF4 (Source: Uniprot.org, NCBI)
Gene Name
HSF4
Full Name
Heat shock factor protein 4
Weight
53011 MW
Superfamily
HSF family
Alternative Names
Heat shock factor protein 4;HSF 4;hHSF4;Heat shock transcription factor 4;HSTF 4;HSF4; HSF4 CTM, CTRCT5 heat shock transcription factor 4 heat shock factor protein 4|HSF 4|HSTF 4|hHSF4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSF4, check out the HSF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSF4 Antibody Picoband® (PB9634)
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No publications found for PB9634
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HSF4 Antibody Picoband®
Question
Our lab want to know about to test anti-HSF4 antibody PB9634 on human lower esophagus muscularis layer for research purposes, then I may be interested in using anti-HSF4 antibody PB9634 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Parker
Verified customer
Asked: 2019-07-09
Answer
The products we sell, including anti-HSF4 antibody PB9634, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-09
Question
We are currently using anti-HSF4 antibody PB9634 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
The anti-HSF4 antibody (PB9634) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lower esophagus muscularis layer using anti-HSF4 antibody PB9634. Let me know if you need anything else.
V. Collins
Verified customer
Asked: 2016-06-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-28
Question
See attached the WB image, lot number and protocol we used for lower esophagus muscularis layer using anti-HSF4 antibody PB9634. Please let me know if you require anything else.
A. Jackson
Verified customer
Asked: 2013-03-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-03-05
