Anti-HSF1 Monoclonal Antibody

Product Name

Anti-HSF1 Monoclonal Antibody

View all HSF1 Antibodies

SKU/Catalog Number

M00250

Size

100ug

Form

liquid

Description

Boster Bio Anti-HSF1 Monoclonal Antibody catalog # M00250. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSF1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00250)

Host

Rat

Contents

PBS pH7.4, 50% glycerol, 0.09% sodium azide

Clonality

Monoclonal

Clone Number

10H8

Isotype

IgG1

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).

Reactive Species

M00250 is reactive to HSF1 in Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat

Reconstitution

Calculated molecular weight

57.26kDa

Background of HSF1

HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the N-terminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00250 is guaranteed for ELISA, IP, IF, IHC, ICC, WB, Gel Shift Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB (1:1000), IHC (1:1000), ICC/IF (1:200); optimal dilutions for assays should be determined by the user.

Validation Images & Assay Conditions

Gene/Protein Information For HSF1 (Source: Uniprot.org, NCBI)

Alternative Names

HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1 HSF1 HSTF1 heat shock transcription factor 1 heat shock factor protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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