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Product Info Summary
| SKU: | A03736-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SET1/SETD1A Antibody Picoband®
SKU/Catalog Number
A03736-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SET1/SETD1A Antibody Picoband® catalog # A03736-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SET1/SETD1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03736-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SET1/SETD1A recombinant protein (Position: D8-K153).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03736-1 is reactive to SETD1A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
186 kDa
Calculated molecular weight
186.034kDa
Background of SETD1A
Histone-lysine N-methyltransferase SETD1A is an enzyme that in humans is encoded by the SETD1A gene. It is mapped to 16p11.2. The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03736-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human SW620 whole cell, human U87 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SET1/SETD1A using anti-SET1/SETD1A antibody (A03736-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SW620 whole cell lysates,
Lane 2: human U87 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SET1/SETD1A antigen affinity purified polyclonal antibody (Catalog # A03736-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SET1/SETD1A at approximately 186KD. The expected band size for SET1/SETD1A is at 186KD.
Protein Target Info & Infographic
Gene/Protein Information For SETD1A (Source: Uniprot.org, NCBI)
Gene Name
SETD1A
Full Name
Histone-lysine N-methyltransferase SETD1A
Weight
186.034kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
Histone-lysine N-methyltransferase SETD1A; Lysine N-methyltransferase 2F; SET domain-containing protein 1A; Hset1a; Set1/Ash2 histone methyltransferase complex subunit SET1; SETD1A; KIAA0339; KMT2F; SET1; SET1A SETD1A EPEDD, KMT2F, NEDSID, Set1, Set1A SET domain containing 1A, histone lysine methyltransferase histone-lysine N-methyltransferase SETD1A|SET domain-containing protein 1A|hSET1A|lysine N-methyltransferase 2F|set1/Ash2 histone methyltransferase complex subunit SET1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SETD1A, check out the SETD1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SETD1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SET1/SETD1A Antibody Picoband® (A03736-1)
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