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Product Info Summary
| SKU: | A04128-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-HSD17B3 Antibody Picoband®
SKU/Catalog Number
A04128-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD17B3 Antibody Picoband® catalog # A04128-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-HSD17B3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04128-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human HSD17B3, which shares 68.4% amino acid (aa) sequence identity with rat HSD17B3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04128-3 is reactive to HSD17B3 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
211344 MW
Background of HSD17B3
17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) is an enzyme that in humans is encoded by the HSD17B3 gene and is involved in androgen steroidogenesis. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04128-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human U20S whole cell, human HEL whole cell, rat testis tissue, rat liver tissue, mouse liver tissue, mouse RAW2647 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HSD17B3 using anti-HSD17B3 antibody (A04128-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: rat testis tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSD17B3 antigen affinity purified polyclonal antibody (Catalog # A04128-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSD17B3 at approximately 40 kDa. The expected band size for HSD17B3 is at 40 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-HSD17B3 antibody (A04128-3).
Overlay histogram showing HepG2 cells stained with A04128-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSD17B3 Antibody (A04128-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B3 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B3
Full Name
Testosterone 17-beta-dehydrogenase 3
Weight
211344 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
Sodium channel protein type 11 subunit alpha; Peripheral nerve sodium channel 5; PN5; Sensory neuron sodium channel 2; Sodium channel protein type XI subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.9; hNaN; SCN11A; SCN12A, SNS2 HSD17B3 EDH17B3, SDR12C2 hydroxysteroid 17-beta dehydrogenase 3 testosterone 17-beta-dehydrogenase 3|17-beta-HSD 3|17-beta-HSD3|17-beta-hydroxysteroid dehydrogenase type 3|estradiol 17-beta-dehydrogenase 2|hydroxysteroid dehydrogenase 3|short chain dehydrogenase/reductase family 12C member 2|testicular 17-beta-hydroxysteroid dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B3, check out the HSD17B3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD17B3 Antibody Picoband® (A04128-3)
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