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Product Info Summary
| SKU: | PA1935 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®
View all Hsp70 interacting protein HIP Antibodies
SKU/Catalog Number
PA1935
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSC70 Interacting Protein HIP/ST13 Antibody catalog # PA1935. Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1935)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human ST13, different from the related rat and mouse sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1935 is reactive to ST13 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45-54 kDa
Calculated molecular weight
41332 MW
Background of Hsp70 interacting protein HIP
ST13 (Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene. ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex. Zhang et al. (1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization. They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site. Using a yeast 2-hybrid assay, Hohfeld et al. (1995) showed that rat Hip bound Hsc70 (HSPA8). One Hip oligomer bound the ATPase domains of at least 2 Hsc70 molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1). Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate. Hohfeld et al. (1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1935 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell, human SKOV3 whole cell, mouse testis tissue, mouse NIH/3T3 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ST13 using anti-ST13 antibody (PA1935).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human SKOV3 whole cell lysates,
Lane 4: mouse testis tissue lysates,
Lane 5: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST13 antigen affinity purified polyclonal antibody (Catalog # PA1935) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST13 at approximately 45-54 kDa. The expected band size for ST13 is at 41 kDa.
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Figure 2. IF analysis of ST13 using anti-ST13 antibody (PA1935).
ST13 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-ST13 Antibody (PA1935) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For ST13 (Source: Uniprot.org, NCBI)
Gene Name
ST13
Full Name
Hsc70-interacting protein
Weight
41332 MW
Superfamily
FAM10 family
Alternative Names
Hsc70-interacting protein;Hip;Aging-associated protein 2;Progesterone receptor-associated p48 protein;Protein FAM10A1;Putative tumor suppressor ST13;Renal carcinoma antigen NY-REN-33;Suppression of tumorigenicity 13 protein;ST13;AAG2, FAM10A1, HIP, SNC6; ST13 AAG2, FAM10A1, FAM10A4, HIP, HOP, HSPABP, HSPABP1, P48, PRO0786, SNC6 ST13 Hsp70 interacting protein hsc70-interacting protein|Hsp70-interacting protein|aging-associated protein 2|heat shock 70kD protein binding protein|progesterone receptor-associated p48 protein|putative tumor suppressor ST13|renal carcinoma NY-REN-33|suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)|suppression of tumorigenicity 13 protein|testis secretory sperm-binding protein Li 233m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ST13, check out the ST13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ST13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband® (PA1935)
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Customer Q&As
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5 Customer Q&As for Anti-HSC70 Interacting Protein HIP/ST13 Antibody Picoband®
Question
Would anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935 work for WB with skin testis?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
According to the expression profile of skin testis, ST13 is highly expressed in skin testis. So, it is likely that anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935 will work for WB with skin testis.
Boster Scientific Support
Answered: 2020-02-06
Question
We are currently using anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
E. Brown
Verified customer
Asked: 2019-12-20
Answer
The anti-HSC70 Interacting Protein HIP/ST13 antibody (PA1935) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
We want to test anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935 on human skin testis for research purposes, then I may be interested in using anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
The products we sell, including anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-13
Question
Would PA1935 anti-HSC70 Interacting Protein HIP/ST13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
You can see on the product datasheet, PA1935 anti-HSC70 Interacting Protein HIP/ST13 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-22
Question
See below the WB image, lot number and protocol we used for skin testis using anti-HSC70 Interacting Protein HIP/ST13 antibody PA1935. Please let me know if you require anything else.
B. Krishna
Verified customer
Asked: 2015-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-10-09
