Product Info Summary
SKU: | M02780 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02780
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody catalog # M02780. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02780)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DHO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HP1 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02780 is reactive to Cbx5 in Human, Mouse, Rat
Applications
M02780 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
70 kDa, 90 kDa
Calculated molecular weight
22225 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Western blot analysis of HP1 alpha expression in SH-SY5Y cell lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For Cbx5 (Source: Uniprot.org, NCBI)
Gene Name
Cbx5
Full Name
Chromobox protein homolog 5
Weight
22225 MW
Alternative Names
Antigen p25; chromobox homolog 5 (Drosophila HP1 alpha); chromobox homolog 5 (HP1 alpha homolog, Drosophila); chromobox homolog 5; chromobox protein homolog 5; Heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha; HP1 alpha homolog; HP1 alpha; HP1; HP1A; HP1-ALPHA; HP1Hs alpha; HP1Hs-alpha Cbx5|2610029O15Rik, C75991, HP, HP1, Hp1a, Hp1alpha|chromobox 5|chromobox protein homolog 5|HP1 alpha|chromobox homolog 5|heterochromatin protein 1 alpha|heterochromatin protein 1 homolog alpha|mHP1(alpha)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cbx5, check out the Cbx5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cbx5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody (M02780)
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No publications found for M02780
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Customer Q&As
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5 Customer Q&As for Anti-HP1 alpha CBX5 Rabbit Monoclonal Antibody
Question
Will M02780 anti-HP1 alpha Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
You can see on the product datasheet, M02780 anti-HP1 alpha Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-03
Question
Do you have a BSA free version of anti-HP1 alpha Rabbit Monoclonal antibody M02780 available?
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HP1 alpha Rabbit Monoclonal antibody M02780 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-25
Question
Is a blocking peptide available for product anti-HP1 alpha Rabbit Monoclonal antibody (M02780)?
G. Singh
Verified customer
Asked: 2017-08-03
Answer
We do provide the blocking peptide for product anti-HP1 alpha Rabbit Monoclonal antibody (M02780). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-08-03
Question
We are currently using anti-HP1 alpha Rabbit Monoclonal antibody M02780 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
V. Zhang
Verified customer
Asked: 2016-07-18
Answer
The anti-HP1 alpha Rabbit Monoclonal antibody (M02780) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-18
Question
Will anti-HP1 alpha Rabbit Monoclonal antibody M02780 work for IF with cervix carcinoma erythroleukemia?
N. Kulkarni
Verified customer
Asked: 2014-09-19
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CBX5 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-HP1 alpha Rabbit Monoclonal antibody M02780 will work for IF with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2014-09-19