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Product Info Summary
| SKU: | A10857 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Homeobox protein Hox-D8 HOXD8 Antibody
SKU/Catalog Number
A10857
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Homeobox protein Hox-D8 HOXD8 Antibody (Catalog # A10857). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Homeobox protein Hox-D8 HOXD8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10857)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human HOXD8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10857 is reactive to HOXD8 in Human
Reconstitution
Calculated molecular weight
31911 MW
Background of HOXD8
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Kosaki K., Teratology 65:50-62(2002).
Hillier L.W., Nature 434:724-731(2005).
Oliver G., Genes Dev. 3:641-650(1989).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10857 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells, K562 cells and HeLa cells, using HOXD8 antibody A10857.
Protein Target Info & Infographic
Gene/Protein Information For HOXD8 (Source: Uniprot.org, NCBI)
Gene Name
HOXD8
Full Name
Homeobox protein Hox-D8
Weight
31911 MW
Superfamily
Antp homeobox family
Alternative Names
Homeobox protein Hox-D8;Homeobox protein Hox-4E;Homeobox protein Hox-5.4;HOXD8;HOX4E; HOXD8 HOX4, HOX4E, HOX5.4 homeobox D8 homeobox protein Hox-D8|Hox-4.5|homeo box 4E|homeo box D8|homeobox protein 5.4|homeobox protein Hox-4E|homeobox protein Hox-5.4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXD8, check out the HOXD8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Homeobox protein Hox-D8 HOXD8 Antibody (A10857)
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No publications found for A10857
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Customer Q&As
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6 Customer Q&As for Anti-Homeobox protein Hox-D8 HOXD8 Antibody
Question
We are currently using anti-HOXD8 antibody A10857 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The anti-HOXD8 antibody (A10857) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-17
Question
Is a blocking peptide available for product anti-HOXD8 antibody (A10857)?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We do provide the blocking peptide for product anti-HOXD8 antibody (A10857). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-22
Question
Does anti-HOXD8 antibody A10857 work for WB with metanephros?
Verified Customer
Verified customer
Asked: 2019-04-26
Answer
According to the expression profile of metanephros, HOXD8 is highly expressed in metanephros. So, it is likely that anti-HOXD8 antibody A10857 will work for WB with metanephros.
Boster Scientific Support
Answered: 2019-04-26
Question
Is this A10857 anti-HOXD8 antibody reactive to the isotypes of HOXD8?
C. Carter
Verified customer
Asked: 2019-02-18
Answer
The immunogen of A10857 anti-HOXD8 antibody is Synthesized peptide derived from C-terminal of human HOXD8. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-18
Question
I see that the anti-HOXD8 antibody A10857 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-19
Question
Can you help my question with product A10857, anti-HOXD8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
V. Jackson
Verified customer
Asked: 2014-02-12
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10857 anti-HOXD8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-02-12
