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Product Info Summary
| SKU: | PB9218 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-HOXA11 Antibody Picoband®
SKU/Catalog Number
PB9218
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HOXA11 Antibody Picoband® catalog # PB9218. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HOXA11 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9218)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human HOXA11 recombinant protein (Position: H74-R245). Human HOXA11 shares 95% amino acid (aa) sequence identity with mouse HOXA11.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9218 is reactive to HOXA11 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
34486 MW
Background of HOXA11
Homeobox protein Hox-A11 is a protein that in humans is encoded by the HOXA11 gene. It is mapped to 7p15.2. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is involved in the regulation of uterine development and is required for female fertility. HOXA11 is essential for the development of the uterosacral ligaments, it has been found that women with pelvic organ prolapse may have weakened connective tissue due to changes in this signaling pathway. Mutations in this gene can cause radio-ulnar synostosis with amegakaryocytic thrombocytopenia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9218 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: Rat Brain Tissue, Human Placenta Tissue, HLEA Whole Cell, HT1080 Whole Cell, HEPA Whole Cell
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: Recombinant Human HOXA11 Protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD.
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Figure 2. Western blot analysis of HOXA11 using anti-HOXA11 antibody (PB9218).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate,
Lane 2: Human Placenta Tissue Lysate,
Lane 3: HLEA Whole Cell Lysate,
Lane 4: HT1080 Whole Cell Lysate,
Lane 5: HEPA Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody (Catalog # PB9218) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 35KD. The expected band size for HOXA11 is at 35KD.
Click image to see more details
Figure 3. Flow Cytometry analysis of U937 cells using anti-HOXA11 antibody (PB9218).
Overlay histogram showing U937 cells stained with PB9218 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HOXA11 Antibody (PB9218,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For HOXA11 (Source: Uniprot.org, NCBI)
Gene Name
HOXA11
Full Name
Homeobox protein Hox-A11
Weight
34486 MW
Superfamily
Abd-B homeobox family
Alternative Names
Homeobox protein Hox-A11;Homeobox protein Hox-1I;HOXA11;HOX1I; HOXA11 HOX1, HOX1I, RUSAT1 homeobox A11 homeobox protein Hox-A11|homeo box 1I|homeobox protein HOXA11|homeobox protein Hox-1I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HOXA11, check out the HOXA11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HOXA11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HOXA11 Antibody Picoband® (PB9218)
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Customer Q&As
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4 Customer Q&As for Anti-HOXA11 Antibody Picoband®
Question
Can you help my question with product PB9218, anti-HOXA11 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9218 anti-HOXA11 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-07
Question
We are currently using anti-HOXA11 antibody PB9218 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The anti-HOXA11 antibody (PB9218) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-26
Question
I see that the anti-HOXA11 antibody PB9218 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-13
Question
Will PB9218 anti-HOXA11 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
As indicated on the product datasheet, PB9218 anti-HOXA11 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-03-05
