Anti-hnRNP U Monoclonal Antibody

hnRNP U antibody

Boster Bio Anti-hnRNP U Monoclonal Antibody catalog # M03691. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M03691
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-hnRNP U Monoclonal Antibody

View all hnRNP U Antibodies

SKU/Catalog Number

M03691

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-hnRNP U Monoclonal Antibody catalog # M03691. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-hnRNP U Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03691)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AEDD-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human hnRNP U Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03691 is reactive to HNRNPU in Human, Mouse, Rat

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

47 kDa

Calculated molecular weight

103854 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03691 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:20

Positive Control

WB: K562 cell

Validation Images & Assay Conditions

Gene/Protein Information For HNRNPU (Source: Uniprot.org, NCBI)

Gene Name

HNRNPU

Full Name

Heterogeneous nuclear ribonucleoprotein U

Weight

103854 MW

Alternative Names

Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; HNRNPU DEE54, EIEE54, GRIP120-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120, HNRNPU heterogeneous nuclear ribonucleoprotein U heterogeneous nuclear ribonucleoprotein U|HNRNPU antisense RNA 1|heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)|nuclear p120 ribonucleoprotein|p120 nuclear protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HNRNPU, check out the HNRNPU Infographic

HNRNPU infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HNRNPU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-hnRNP U Monoclonal Antibody

Question

Will anti-hnRNP U Monoclonal antibody M03691 work for IF with ovarian carcinoma?

M. Jha

Verified customer

Asked: 2019-07-23

Answer

According to the expression profile of ovarian carcinoma, HNRNPU is highly expressed in ovarian carcinoma. So, it is likely that anti-hnRNP U Monoclonal antibody M03691 will work for IF with ovarian carcinoma.

Boster Scientific Support

Answered: 2019-07-23

Question

My question regarding product M03691, anti-hnRNP U Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Carter

Verified customer

Asked: 2018-06-11

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03691 anti-hnRNP U Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-06-11

Question

We are currently using anti-hnRNP U Monoclonal antibody M03691 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?

Z. Taylor

Verified customer

Asked: 2013-09-19

Answer

The anti-hnRNP U Monoclonal antibody (M03691) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-09-19

Order DetailsPrice
M03691

100μL

$370
M03691-30ul

30μL sample

$99
M03691-carrier-free-1ml

Carrier Free

$2590

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Free Secondary Antibody

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M03691
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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