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Product Info Summary
| SKU: | M03691 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-hnRNP U Monoclonal Antibody
SKU/Catalog Number
M03691
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-hnRNP U Monoclonal Antibody catalog # M03691. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-hnRNP U Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03691)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEDD-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human hnRNP U Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03691 is reactive to HNRNPU in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
103854 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03691 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:20
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of hnRNP U expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HNRNPU (Source: Uniprot.org, NCBI)
Gene Name
HNRNPU
Full Name
Heterogeneous nuclear ribonucleoprotein U
Weight
103854 MW
Alternative Names
Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; HNRNPU DEE54, EIEE54, GRIP120-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp120, HNRNPU heterogeneous nuclear ribonucleoprotein U heterogeneous nuclear ribonucleoprotein U|HNRNPU antisense RNA 1|heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)|nuclear p120 ribonucleoprotein|p120 nuclear protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HNRNPU, check out the HNRNPU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HNRNPU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-hnRNP U Monoclonal Antibody (M03691)
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No publications found for M03691
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Customer Q&As
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3 Customer Q&As for Anti-hnRNP U Monoclonal Antibody
Question
Will anti-hnRNP U Monoclonal antibody M03691 work for IF with ovarian carcinoma?
M. Jha
Verified customer
Asked: 2019-07-23
Answer
According to the expression profile of ovarian carcinoma, HNRNPU is highly expressed in ovarian carcinoma. So, it is likely that anti-hnRNP U Monoclonal antibody M03691 will work for IF with ovarian carcinoma.
Boster Scientific Support
Answered: 2019-07-23
Question
My question regarding product M03691, anti-hnRNP U Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Carter
Verified customer
Asked: 2018-06-11
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03691 anti-hnRNP U Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-11
Question
We are currently using anti-hnRNP U Monoclonal antibody M03691 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Z. Taylor
Verified customer
Asked: 2013-09-19
Answer
The anti-hnRNP U Monoclonal antibody (M03691) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-09-19
