Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

Product Name

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3)

View all HLA-DQA1 Antibodies

SKU/Catalog Number

M00232

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3) (Catalog# M00232). Tested in FACS application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-hMHCII/HLA DQ Antibody (Monoclonal, SPVL3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00232)

Host

Mouse

Contents

100 μg in 200 μl PBS containing 0.05% BSA and 0.05% sodium azide.

Clonality

Monoclonal

Clone Number

SPVL3

Isotype

IgG2a Kappa

Immunogen

Cells of T4 and CTL clone HG-38

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00232 is reactive to HLA-DQA1 in Human

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

27805 MW

Background of HLA-DQA1

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00232 is guaranteed for FACS Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FACS: 0.5-1 µg/10^6 cells

Validation Images & Assay Conditions

Gene/Protein Information For HLA-DQA1 (Source: Uniprot.org, NCBI)

Alternative Names

HLA class II histocompatibility antigen, DQ alpha 1 chain;DC-1 alpha chain;DC-alpha;HLA-DCA;MHC class II DQA1;HLA-DQA1; HLA-DQA1 CELIAC1, DQ-A1, DQA1, HLA-DQA major histocompatibility complex, class II, DQ alpha 1 HLA class II histocompatibility , DQ alpha 1 chain|DC-1 alpha chain|DC-alpha|HLA class II histocompatibility DQ alpha chain|HLA-DCA|MHC HLA-DQ alpha|MHC class II DQ alpha chain|MHC class II DQA1|MHC class II HLA-DQ-alpha-1|MHC class II DQA1|MHC class II protein|truncated MHC class II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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