IL-1-beta Interleukin-1 beta Porcine Recombinant Protein

IL-1 beta/IL-1F2 protein, Pig

Recombinant IL 1 beta Porcine produced in E.coli cells is a non-glycosylated, homodimeric protein containing 153 amino acid chain and having a molecular mass of 17.6kDa. The IL 1 beta is purified by proprietary chromatographic techniques.

Product Info Summary

SKU: PROTP26889
Size: 2ug, 10ug, 1mg
Origin Species: Pig
Source: Escherichia coli

Product Name

IL-1-beta Interleukin-1 beta Porcine Recombinant Protein

View all IL-1 beta/IL-1F2 recombinant proteins

SKU/Catalog Number

PROTP26889

Size

2ug, 10ug, 1mg

Description

Recombinant IL 1 beta Porcine produced in E.coli cells is a non-glycosylated, homodimeric protein containing 153 amino acid chain and having a molecular mass of 17.6kDa. The IL 1 beta is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized IL 1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL 1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Cite This Product

IL-1-beta Interleukin-1 beta Porcine Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP26889)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The IL 1 beta was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

30.748kDa

Reconstitution

It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Amino Acid Sequence

ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP

Biological Activity

The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of greater than 2.0 × 105 IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For IL1B (Source: Uniprot.org, NCBI)

Gene Name

IL1B

Full Name

Interleukin-1 beta

Weight

30.748kDa

Superfamily

IL-1 family

Alternative Names

Catabolin; Lymphocyte-activating factor (LAF); Endogenous Pyrogen (EP); Leukocyte Endogenous Mediator (LEM); Mononuclear Cell Factor (MCF); IL1F2; IL-1 beta IL1B IL-1, IL1-BETA, IL1F2, IL1beta interleukin 1 beta interleukin-1 beta|IL-1 beta|catabolin|interleukin 1beta|preinterleukin 1 beta|pro-interleukin-1-beta

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

For more info on IL1B, check out the IL1B Infographic

IL1B infographic

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In this infographic, you will see the following information for IL1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us at [email protected].

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Size

Total: $250

SKU:PROTP26889

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