Click image to see more details
Product Info Summary
| SKU: | PB9375 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HKDC1 Antibody Picoband®
SKU/Catalog Number
PB9375
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HKDC1 Antibody Picoband® catalog # PB9375. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HKDC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9375)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HKDC1, different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9375 is reactive to HKDC1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
103 kDa
Calculated molecular weight
102545 MW
Background of HKDC1
The epidermal growth factor receptor (HKDC1; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: HKDC1 (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). HKDC1 exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα). HKDC1 and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to HKDC1 overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of HKDC1, called HKDC1vIII is often observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9375 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human 293T whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of HKDC1 using anti-HKDC1 antibody (PB9375).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HKDC1 antigen affinity purified polyclonal antibody (Catalog # PB9375) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HKDC1 at approximately 103 kDa. The expected band size for HKDC1 is at 103 kDa.
Protein Target Info & Infographic
Gene/Protein Information For HKDC1 (Source: Uniprot.org, NCBI)
Gene Name
HKDC1
Full Name
Hexokinase HKDC1
Weight
102545 MW
Superfamily
hexokinase family
Alternative Names
Putative hexokinase HKDC1;2.7.1.1;Hexokinase domain-containing protein 1;HKDC1; HKDC1 hexokinase domain containing 1 hexokinase HKDC1|hexokinase domain-containing protein 1|putative hexokinase HKDC1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HKDC1, check out the HKDC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HKDC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HKDC1 Antibody Picoband® (PB9375)
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Customer Q&As
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5 Customer Q&As for Anti-HKDC1 Antibody Picoband®
Question
Does anti-HKDC1 antibody PB9375 work on canine WB with liver?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-HKDC1 antibody PB9375 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HKDC1 antibody PB9375 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
I see that the anti-HKDC1 antibody PB9375 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-HKDC1 antibody PB9375 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
V. Parker
Verified customer
Asked: 2016-05-06
Answer
The anti-HKDC1 antibody (PB9375) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-05-06
Question
I have a question about product PB9375, anti-HKDC1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
N. Rodriguez
Verified customer
Asked: 2015-04-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9375 anti-HKDC1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-15
Question
See below the WB image, lot number and protocol we used for ileal mucosa using anti-HKDC1 antibody PB9375. Please let me know if you require anything else.
A. Gonzalez
Verified customer
Asked: 2014-11-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-07
