Product Info Summary
SKU: | M14495-4 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody
SKU/Catalog Number
M14495-4
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody catalog # M14495-4. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M14495-4)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CCA-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H4 (acetyl K5) Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4) . The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M14495-4 is reactive to HIST1H4A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
11 kDa
Calculated molecular weight
11367 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M14495-4 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: Untreated HeLa HeLa cell, TSA treated HeLa cell
IHC: human colon tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Histone H4 (acetyl K5) expression in (1) Untreated HeLa HeLa cell lysate; (2) TSA treated HeLa cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human colon, using Histone H4 (acetyl K5) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H4A (Source: Uniprot.org, NCBI)
Gene Name
HIST1H4A
Full Name
Histone H4
Weight
11367 MW
Superfamily
histone H4 family
Alternative Names
Histone H4;HIST1H4A;H4/A, H4FA;HIST1H4B;H4/I, H4FI;HIST1H4C;H4/G, H4FG;HIST1H4D;H4/B, H4FB;HIST1H4E;H4/J, H4FJ;HIST1H4F;H4/C, H4FC;HIST1H4H;H4/H, H4FH;HIST1H4I;H4/M, H4FM;HIST1H4J;H4/E, H4FE;HIST1H4K;H4/D, H4FD;HIST1H4L;H4/K, H4FK;HIST2H4A;H4/N, H4F2, H4FN, HIST2H4;HIST2H4B;H4/O, H4FO;HIST4H4; H4C1 H4-161, H4C12, H4C13, H4C14, H4C15, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A, H4C1 H4 clustered histone 1 histone H4|H4 histone family, member A|histone 1, H4a|histone cluster 1 H4 family member a|histone cluster 1, H4a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H4A, check out the HIST1H4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H4 (acetyl K5) HIST1H4A Monoclonal Antibody (M14495-4)
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