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Product Info Summary
| SKU: | P12718 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody
View all Histone H2B type 1B Antibodies
SKU/Catalog Number
P12718
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody catalog # P12718. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P12718)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AC-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P12718 is reactive to HIST1H2BB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
140 kDa, 130 kDa, 110 kDa
Calculated molecular weight
13950 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P12718 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:20000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:10
FC 1:10
Positive Control
WB: Hela cell
IHC: human colon tissue
Validation Images & Assay Conditions
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Western blot analysis of Histone H2B (acetyl K20) expression in Hela cell treated with TSA lysate.
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Immunohistochemical analysis of paraffin-embedded human colon, using Histone H2B (acetyl K20) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H2BB (Source: Uniprot.org, NCBI)
Gene Name
HIST1H2BB
Full Name
Histone H2B type 1-B
Weight
13950 MW
Superfamily
histone H2B family
Alternative Names
Histone H2B type 1-B;Histone H2B.1;Histone H2B.f;H2B/f;HIST1H2BB;H2BFF;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H2BB, check out the HIST1H2BB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H2BB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody (P12718)
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No publications found for P12718
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Histone H2B (acetyl K20) HIST1H2BB Rabbit Monoclonal Antibody
Question
Here is the WB image, lot number and protocol we used for lung using anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
Would anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 work for IF with lung?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
According to the expression profile of lung, HIST1H2BB is highly expressed in lung. So, it is likely that anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 will work for IF with lung.
Boster Scientific Support
Answered: 2020-02-05
Question
I have a question about product P12718, anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P12718 anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-25
Question
I see that the anti-Histone H2B (acetyl K20) Rabbit Monoclonal antibody P12718 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-18
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-18
