Product Info Summary
SKU: | M16777 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody
SKU/Catalog Number
M16777
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody catalog # M16777. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EOO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2A.Z
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M16777 is reactive to H2afz in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
14135 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16777 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: Neuro-2a cell,HeLa cell
IHC: human kidney tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Histone H2A.Z expression in (1)Neuro-2a cell lysate;(2)HeLa cell lysate.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using Histone H2A.Z Antibody.
Protein Target Info & Infographic
Gene/Protein Information For H2afz (Source: Uniprot.org, NCBI)
Gene Name
H2afz
Full Name
Histone H2A.Z
Weight
14135 MW
Superfamily
histone H2A family
Alternative Names
Histone H2A type 1-B/E;Histone H2A.2;Histone H2A/a;Histone H2A/m;HIST1H2AB;H2AFM;HIST1H2AE;H2AFA; H2AZ1 H2A.Z-1, H2A.z, H2A/z, H2AFZ, H2AZ H2A.Z variant histone 1 histone H2A.Z|H2A histone family member Z|H2AZ histone
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on H2afz, check out the H2afz Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for H2afz: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody (M16777)
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No publications found for M16777
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Customer Q&As
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5 Customer Q&As for Anti-Histone H2A.Z H2afz Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 available?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-15
Question
We are currently using anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-Histone H2A.Z Rabbit Monoclonal antibody (M16777) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
My question regarding product M16777, anti-Histone H2A.Z Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M16777 anti-Histone H2A.Z Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-04
Question
Would anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 work on horse IHC with brain?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
Our lab technicians have not tested anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Histone H2A.Z Rabbit Monoclonal antibody M16777 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-24
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Histone H2A.Z Rabbit Monoclonal antibody M16777. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-06-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-06-25