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Product Info Summary
| SKU: | M16777-3 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody
SKU/Catalog Number
M16777-3
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody catalog # M16777-3. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M16777-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DEB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Histone H2A (acetyl K9) Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M16777-3 is reactive to HIST1H2AB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
37 kDa
Calculated molecular weight
14135 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M16777-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:500-1:1000
ICC/IF 1:500-1:1000
Positive Control
WB: HeLa cell
IHC: mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of Histone H2A (acetyl K9) expression in HeLa cell lysate treated Trichostatin A.
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Immunohistochemical analysis of paraffin-embedded mouse brain, using Histone H2A (acetyl K9) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIST1H2AB (Source: Uniprot.org, NCBI)
Gene Name
HIST1H2AB
Full Name
Histone H2A type 1-B/E
Weight
14135 MW
Superfamily
histone H2A family
Alternative Names
Histone H2A type 1-B/E;Histone H2A.2;Histone H2A/a;Histone H2A/m;HIST1H2AB;H2AFM;HIST1H2AE;H2AFA; H2AC4 H2A/m, H2AC8, H2AFM, HIST1H2AB H2A clustered histone 4 histone H2A type 1-B/E|H2A histone family, member M|histone 1, H2ab|histone H2A/m|histone cluster 1 H2A family member b|histone cluster 1, H2ab
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST1H2AB, check out the HIST1H2AB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST1H2AB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Histone H2A (acetyl K9) HIST1H2AB Monoclonal Antibody (M16777-3)
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