Click image to see more details
Product Info Summary
| SKU: | M30975-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | All species |
| Host: | Mouse |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-His Tag Antibody Picoband® (monoclonal, 19b10)
SKU/Catalog Number
M30975-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-His Tag Antibody Picoband® (monoclonal, 19b10) catalog # M30975-1. Tested in WB application. This antibody reacts with all species. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-His Tag Antibody Picoband® (monoclonal, 19b10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30975-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
19b10
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence of His Tag.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M30975-1 is reactive to GNG4 in All species
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
8.389kDa
Background of G gamma4
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4 is a protein that in humans is encoded by the GNG4 gene. This gene encodes the gamma subunit of the heterotrimeric G-proteins that are comprised of alpha, beta and gamma subunits. Upon activation by G protein-coupled receptors, the beta-gamma heterodimer dissociates from the alpha subunit to activate downstream signaling events. Alternate splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M30975-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of His Tag using anti-His Tag antibody (M30975-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant mouse His Tag protein 10ng,
Lane 1: recombinant mouse His Tag protein 5ng,
Lane 1: recombinant mouse His Tag protein 2.5ng,
Lane 1: recombinant mouse His Tag protein 1.25ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-His Tag antigen affinity purified monoclonal antibody (Catalog # M30975-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For GNG4 (Source: Uniprot.org, NCBI)
Gene Name
GNG4
Full Name
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4
Weight
8.389kDa
Superfamily
G protein gamma family
Alternative Names
ATP synthase D chain mitochondrial antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit antibody|ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody|ATP synthase subunit d antibody|ATP synthase subunit d, mitochondrial antibody|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody|ATP5H antibody|ATP5H_HUMAN antibody|ATP5JD antibody|ATPase subunit d antibody|ATPQ antibody|mitochondrial antibody|My032 protein antibody GNG4 G protein subunit gamma 4 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-4|guanine nucleotide binding protein (G protein), gamma 4|guanine nucleotide binding protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNG4, check out the GNG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-His Tag Antibody Picoband® (monoclonal, 19b10) (M30975-1)
Hello CJ!
M30975-1 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Molecular structure of the largemouth bass (Micropterus salmoides) Myf5 gene and its effect on skeletal muscle growth
Li L,Yu X,Zhang H,Cheng H,Hou L,Zheng Q,Hou J.In vitro antiviral activity of Griffithsin against porcine epidemic diarrhea virus.Virus Genes.2019 Apr;55(2):174-181.doi:10.1007/s11262-019-01633-7.Epub 2019 Jan 14.PMID:30637608;PMCID:PMC7089098.
Species: Monkey
M30975-1 usage in article: APP:ELISA, SAMPLE:VERO CELL, DILUTION:NA
Wang J,Li Y,Pan L,Li J,Yu Y,Liu B,Zubair M,Wei Y,Pillay B,Olaniran AO,Chiliza TE,Shao G,Feng Z,Xiong Q.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation.Vet Res.2021 Jun 3;52(1):80.doi:10.1186/s13567-021-00952-8. PMID:34082810;PMCID:PMC8173509.
Species: Human
M30975-1 usage in article: APP:PROTEIN QUANTITATION, SAMPLE:E COLI CELL, DILUTION:1:1000
Gao-Yang Liu,Jian Li,Zhong-Yu Zhang,Jing-Yao Liu,Chen Zhang,Bin Zhu,Gao-Xue Wang,An immersion subunit vaccine loaded by single-walled carbon nanotube protects pearl gentian grouper from viral nervous necrosis virus,Aquaculture,Volume 541,2021,736813,ISSN 0044-8486,https://doi.org/10.1016/j.aquaculture.2021. 736813.
Species: Snakehead fish
M30975-1 usage in article: APP:WB, SAMPLE:SSN-1 CELL, DILUTION:NA
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-His Tag Antibody Picoband® (monoclonal, 19b10)?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-His Tag Antibody Picoband® (monoclonal, 19b10)
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
