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Product Info Summary
| SKU: | A12886 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-HIPK4 Antibody
SKU/Catalog Number
A12886
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-HIPK4 Antibody (Catalog # A12886). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIPK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12886)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human HIPK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12886 is reactive to HIPK4 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
69425 MW
Background of HIPK4
Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter By similarity. May act as a corepressor of transcription factors Potential.
Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12886 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human testis tissue using HIPK4 antibody A12886.
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Western blot analysis of extracts from HUVEC cells, using HIPK4 antibody A12886.
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Immunofluorescence analysis of A549 cells, using HIPK4 antibody A12886.
Protein Target Info & Infographic
Gene/Protein Information For HIPK4 (Source: Uniprot.org, NCBI)
Gene Name
HIPK4
Full Name
Homeodomain-interacting protein kinase 4
Weight
69425 MW
Superfamily
protein kinase superfamily
Alternative Names
Homeodomain-interacting protein kinase 4;2.7.11.1;HIPK4; HIPK4 homeodomain interacting protein kinase 4 homeodomain-interacting protein kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIPK4, check out the HIPK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIPK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIPK4 Antibody (A12886)
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No publications found for A12886
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-HIPK4 Antibody
Question
I am looking for to test anti-HIPK4 antibody A12886 on human testis for research purposes, then I may be interested in using anti-HIPK4 antibody A12886 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
The products we sell, including anti-HIPK4 antibody A12886, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-02
Question
I see that the anti-HIPK4 antibody A12886 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-04
Question
We are currently using anti-HIPK4 antibody A12886 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
The anti-HIPK4 antibody (A12886) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-06
Question
I was wanting to use your anti-HIPK4 antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can see on the product datasheet, A12886 anti-HIPK4 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-20
Question
Is this A12886 anti-HIPK4 antibody reactive to the isotypes of HIPK4?
G. Anderson
Verified customer
Asked: 2016-06-02
Answer
The immunogen of A12886 anti-HIPK4 antibody is Synthesized peptide derived from internal of human HIPK4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-06-02
