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Product Info Summary
| SKU: | M02116-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, WB |
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Product info
Product Name
Anti-HIF1AN Antibody (C-term)
View all FIH-1/HIF-1AN Antibodies
SKU/Catalog Number
M02116-1
Size
50 µl
Description
Boster Bio Anti-HIF1AN Antibody (C-term) (Catalog # M02116-1). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-HIF1AN Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02116-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
931CT3.1.1
Isotype
IgG1,k
Immunogen
This HIF1AN antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-349 amino acids from human HIF1AN.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02116-1 is reactive to HIF1AN in Human
Applications
M02116-1 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
40285 Da
Background of FIH-1/HIF-1AN
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with M02116-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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All lanes : Anti-HIF1AN Antibody (C-term) at 1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: ZR-75-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 40 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For HIF1AN (Source: Uniprot.org, NCBI)
Gene Name
HIF1AN
Full Name
Hypoxia-inducible factor 1-alpha inhibitor
Weight
40285 Da
Alternative Names
DKFZp762F1811; EC 1.14.11.16; factor inhibiting HIF1; Factor inhibiting HIF-1; FIH1; FIH-1; FIH1FIH-1; FLJ20615; FLJ22027; HIF1AN; HIF-1AN; hypoxia inducible factor 1, alpha subunit inhibitor; hypoxia-inducible factor 1-alpha inhibitor; Hypoxia-inducible factor asparagine hydroxylase; Peptide-aspartate beta-dioxygenase HIF1AN FIH1 hypoxia inducible factor 1 subunit alpha inhibitor hypoxia-inducible factor 1-alpha inhibitor|FIH-1|factor inhibiting HIF-1|factor inhibiting HIF1|hypoxia inducible factor 1 alpha subunit inhibitor|hypoxia-inducible factor asparagine hydroxylase|peptide-aspartate beta-dioxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIF1AN, check out the HIF1AN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIF1AN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF1AN Antibody (C-term) (M02116-1)
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