Anti-HIBADH Antibody Picoband™

Product Name

Anti-HIBADH Antibody Picoband™

View all HIBADH Antibodies

SKU/Catalog Number

A08760-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HIBADH Antibody Picoband™ catalog # A08760-3. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-HIBADH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08760-3)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human HIBADH recombinant protein (Position: R34-E332). Human HIBADH shares 93.3% and 92.6% amino acid (aa) sequence identity with mouse and rat HIBADH, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08760-3 is reactive to HIBADH in Human, Mouse, Rat

Applications

A08760-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

Background of HIBADH

In enzymology, a 3-hydroxyisobutyrate dehydrogenase also known as β-hydroxyisobutyrate dehydrogenase or 3-hydroxyisobutyrate dehydrogenase, mitochondrial (HIBADH) is an enzyme that in humans is encoded by the HIBADH gene. This gene encodes a mitochondrial 3-hydroxyisobutyrate dehydrogenase enzyme. The encoded protein plays a critical role in the catabolism of L-valine by catalyzing the oxidation of 3-hydroxyisobutyrate to methylmalonate semialdehyde.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HIBADH using anti-HIBADH antibody (A08760-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human SIHA whole cell lysates,
Lane 5: rat heart tissue lysates,
Lane 6: rat NRK whole cell lysates,
Lane 7: mouse heart tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIBADH antigen affinity purified polyclonal antibody (Catalog # A08760-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIBADH at approximately 32 kDa. The expected band size for HIBADH is at 35 kDa.

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Figure 2. Flow Cytometry analysis of A549 cells using anti-HIBADH antibody (A08760-3).
Overlay histogram showing A549 cells stained with A08760-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIBADH Antibody (A08760-3, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For HIBADH (Source: Uniprot.org, NCBI)

Alternative Names

3-hydroxyisobutyrate dehydrogenase; 3-hydroxyisobutyrate dehydrogenase, mitochondrial; EC 1.1.1,3'-hydroxyisobutyrate dehydrogenase; EC 1.1.1.31; MGC40361; NS5ATP1 HIBADH NS5ATP1 3-hydroxyisobutyrate dehydrogenase 3-hydroxyisobutyrate dehydrogenase, mitochondrial|3-hydroxyisobutyrate dehydrogenase|epididymis secretory sperm binding protein

For more info on HIBADH, check out the HIBADH Infographic

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In this infographic, you will see the following information for HIBADH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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