Anti-Histone deacetylase 5 HDAC5 Antibody Picoband®

Product Name

Anti-Histone deacetylase 5 HDAC5 Antibody Picoband®

View all HDAC5 Antibodies

SKU/Catalog Number

PA2180

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Histone deacetylase 5 HDAC5 Antibody catalog # PA2180. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Histone deacetylase 5 HDAC5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2180)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC5, different from the related rat and mouse sequences by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2180 is reactive to HDAC5 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

53 kDa

Calculated molecular weight

121978 MW

Background of HDAC5

Histone deacetylase 5, also called HDAC5 or KIAA0600, is an enzyme that in humans is encodes by the HDAC5 gene. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. This gene is mapped to 17q21.31. Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is though to be associated with colon cancer.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2180 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

WB: HELA Cell, COLO320 Cell

Validation Images & Assay Conditions

Gene/Protein Information For HDAC5 (Source: Uniprot.org, NCBI)

Alternative Names

Histone deacetylase 5;HD5;3.5.1.98;Antigen NY-CO-9;HDAC5;KIAA0600; HDAC5 HD5, NY-CO-9 histone deacetylase 5 histone deacetylase 5| NY-CO-9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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