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Product Info Summary
| SKU: | M02830 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HBG1/2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02830
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HBG1/2 Rabbit Monoclonal Antibody catalog # M02830. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HBG1/2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02830)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AADB-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HBG1/2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02830 is reactive to HBG1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
16140 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02830 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of HBG1/2 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For HBG1 (Source: Uniprot.org, NCBI)
Gene Name
HBG1
Full Name
Hemoglobin subunit gamma-1
Weight
16140 MW
Superfamily
globin family
Alternative Names
Hemoglobin subunit gamma-1;Gamma-1-globin;Hb F Agamma;Hemoglobin gamma-1 chain;Hemoglobin gamma-A chain;HBG1;PRO2979; HBG1 HBG-T2, HBGA, HBGR, HSGGL1, PRO2979 hemoglobin subunit gamma 1 hemoglobin subunit gamma-1|A-gamma globin|gamma A hemoglobin|gamma globin|gamma-1-globin|hb F Agamma|hemoglobin gamma-1 chain|hemoglobin gamma-a chain|hemoglobin, gamma A|hemoglobin, gamma, regulator of
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HBG1, check out the HBG1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HBG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HBG1/2 Rabbit Monoclonal Antibody (M02830)
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No publications found for M02830
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-HBG1/2 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-HBG1/2 Rabbit Monoclonal antibody for WB for human fetal liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal liver identification?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
It shows on the product datasheet, M02830 anti-HBG1/2 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-02
Question
We are currently using anti-HBG1/2 Rabbit Monoclonal antibody M02830 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
The anti-HBG1/2 Rabbit Monoclonal antibody (M02830) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-11-30
Question
I have attached the WB image, lot number and protocol we used for fetal liver using anti-HBG1/2 Rabbit Monoclonal antibody M02830. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-06-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-06-30
Question
Does M02830 anti-HBG1/2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Baker
Verified customer
Asked: 2014-02-05
Answer
As indicated on the product datasheet, M02830 anti-HBG1/2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-05
