Anti-HA2Q HLA-DPA1 Antibody

Product Name

Anti-HA2Q HLA-DPA1 Antibody

View all HLA DPA Antibodies

SKU/Catalog Number

A02225

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-HA2Q HLA-DPA1 Antibody (Catalog # A02225). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HA2Q HLA-DPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02225)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human HA2Q.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02225 is reactive to HLA-DPA1 in Human

Applications

A02225 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29381 MW

Background of HLA DPA

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Lawrance S.K., Nucleic Acids Res. 13:7515-7528(1985).
Gustafsson K., J. Biol. Chem. 262:8778-8786(1987).
Young J.A., Hum. Immunol. 23:37-44(1988).<

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

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Western blot analysis of extracts from Jurkat cells, using HA2Q antibody A02225.

Gene/Protein Information For HLA-DPA1 (Source: Uniprot.org, NCBI)

Alternative Names

DP alpha 1 chain; HLA class II histocompatibility antigen, DP alpha chain; HLADP; HLA-SB alpha chain; major histocompatibility complex, class II, DP alpha 1; MHC class II antigen; MHC class II DP3-alpha; MHC class II DPA1; MHC class II HLA-DPA1 antigen; Primed lymphocyte test-1 HLA-DPA1 DP(W3), DP(W4), DPA1, HLA-DP1A, HLA-DPA, HLA-DPB1, HLADP, HLASB, PLT1 major histocompatibility complex, class II, DP alpha 1 HLA class II histocompatibility antigen, DP alpha 1 chain|HLA DPA1|HLA-DPA1*01:03:01:New|HLA-DPA1*02:01:01:NEW|HLA-SB alpha chain|MHC class II DP alpha chain|MHC class II DP3-alpha|MHC class II HLA-DPA1 antigen|MHC class II antigen DP alpha chain|MHC class II protein

For more info on HLA-DPA1, check out the HLA-DPA1 Infographic

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In this infographic, you will see the following information for HLA-DPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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