Anti-GTP-binding protein Di-Ras3 DIRAS3 Antibody

Product Name

Anti-GTP-binding protein Di-Ras3 DIRAS3 Antibody

View all DIRAS3 Antibodies

SKU/Catalog Number

A06954

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-GTP-binding protein Di-Ras3 DIRAS3 Antibody (Catalog # A06954). Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

DIRAS3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-GTP-binding protein Di-Ras3 DIRAS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06954)

Host

Rabbit

Contents

DIRAS3 antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

DIRAS3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DIRAS3. The immunogen is located within the last 50 amino acids of DIRAS3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

DIRAS3 antibody is human specific.

Reactive Species

A06954 is reactive to DIRAS3 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

25861 MW

Background of DIRAS3

DIRAS3 is a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with mono-allelic expression of the paternal allele, which is associated with growth suppression and down-regulation of cyclin D1 promoter activity and induction of p21 (WAF/CIP1). Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers (1). DIRAS3 has been shown to induce autophagy in human ovarian cancer cells by blocking PI3K signaling, inhibiting the mammalian target of rapamycin (TOR), upregulating ATG4, and colocalizing with LC3 in autophagosomes (2). DIRAS also interacts with C-RAF and downregulates mitogen-activated protein kinase kinases (MEK) to restrict cell migration (3).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06954 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

DIRAS3 antibody can be used for detection of DIRAS3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DIRAS3 (Source: Uniprot.org, NCBI)

Alternative Names

DIRAS family GTP-binding RAS-like 3, ARHI, NOEY2 DIRAS3 ARHI, NOEY2 DIRAS family GTPase 3 GTP-binding protein Di-Ras3|DIRAS family, GTP-binding RAS-like 3|distinct subgroup of the Ras family member 3|ras homolog gene family, member I|rho-related GTP-binding protein RhoI

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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