Anti-GSK3 alpha/GSK3A Antibody Picoband™

Product Name

Anti-GSK3 alpha/GSK3A Antibody Picoband™

View all GSK3A Antibodies

SKU/Catalog Number

A03152-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GSK3 alpha/GSK3A Antibody Picoband™ catalog # A03152-4. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GSK3 alpha/GSK3A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03152-4)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GSK3 alpha/GSK3A, which shares 76.5% and 88.2% amino acid (aa) sequence identity with mouse and rat GSK3 alpha/GSK3A, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03152-4 is reactive to GSK3A in Human

Applications

A03152-4 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

65411 MW

Background of GSK3A

Glycogen synthase kinase-3 alpha is an enzyme that in humans is encoded by the GSK3A gene. It is mapped to 19q13.2. This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GSK3 alpha/GSK3A using anti-GSK3 alpha/GSK3A antibody (A03152-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha/GSK3A antigen affinity purified polyclonal antibody (Catalog # A03152-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha/GSK3A at approximately 51KD. The expected band size for GSK3 alpha/GSK3A is at 51KD.

Gene/Protein Information For GSK3A (Source: Uniprot.org, NCBI)

Alternative Names

DKFZp686D0638; EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 alpha; glycogen synthase kinase-3 alpha; GSK3 alpha; GSK-3 alpha; GSK3A GSK3A glycogen synthase kinase 3 alpha glycogen synthase kinase-3 alpha|GSK-3 alpha|serine/threonine-protein kinase GSK3A

For more info on GSK3A, check out the GSK3A Infographic

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In this infographic, you will see the following information for GSK3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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