Anti-GPX1 Antibody Picoband® (monoclonal, 8B10)

Glutathione Peroxidase 1/GPX1 antibody

Boster Bio Anti-GPX1 Antibody Picoband® (monoclonal, 8B10) catalog # M01019-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: M01019-2
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-GPX1 Antibody Picoband® (monoclonal, 8B10)

View all Glutathione Peroxidase 1/GPX1 Antibodies

SKU/Catalog Number

M01019-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GPX1 Antibody Picoband® (monoclonal, 8B10) catalog # M01019-2. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPX1 Antibody Picoband® (monoclonal, 8B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01019-2)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

8B10

Isotype

Mouse IgG2b

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01019-2 is reactive to GPX1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Observed Molecular Weight

22 kDa

Calculated molecular weight

22.088kDa

Background of Glutathione Peroxidase 1/GPX1

Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01019-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells

Positive Control

WB: human placenta tissue, human U-87 whole cell, human THP-1 whole cell, human U-937 whole cell, human HepG2 whole cell, rat brain tissue, rat thymus tissue, rat lung tissue, rat liver tissue, mouse brain tissue, mouse thymus tissue, mouse lung tissue, mouse liver tissue
IHC: human lung cancer tissue, human mammary cancer tissue
IF: human colon cancer tissue
FCM: U87 cell, U251 cell

Validation Images & Assay Conditions

Gene/Protein Information For GPX1 (Source: Uniprot.org, NCBI)

Gene Name

GPX1

Full Name

Glutathione peroxidase 1

Weight

22.088kDa

Superfamily

glutathione peroxidase family

Alternative Names

Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1 GPX1 GPXD, GSHPX1 glutathione peroxidase 1 glutathione peroxidase 1|GSHPx-1|cellular glutathione peroxidase|selenoprotein GPX1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPX1, check out the GPX1 Infographic

GPX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
M01019-2

100μg

$370
M01019-2-10ug

10μg sample (liquid)

$99
M01019-2-Biotin

100 μg Biotin conjugated

$570
M01019-2-Cy3

100 μg Cy3 conjugated

$570
M01019-2-Dylight488

100 μg Dylight488 conjugated

$570
M01019-2-Dylight550

100 μg Dylight550 conjugated

$570
M01019-2-Dylight594

100 μg Dylight594 conjugated

$570
M01019-2-FITC

100 μg FITC conjugated

$570
M01019-2-HRP

100 μg HRP conjugated

$570
M01019-2-APC

100 μg APC conjugated

$670
M01019-2-PE

100 μg PE conjugated

$670
M01019-2-iFluor647

100 μg iFluor647 conjugated

$670
M01019-2-carrier-free

Carrier Free

$370

More conjugates/formats

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M01019-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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