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Product Info Summary
| SKU: | A10818 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-GPR12/Gpcr12 Antibody
SKU/Catalog Number
A10818
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GPR12/Gpcr12 Antibody (Catalog# A10818). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GPR12/Gpcr12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10818)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human GPR12. Immunogen sequence location: 18264
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10818 is reactive to GPR12 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
36730 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10818 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunofluorescence analysis of A549 cells, using GPR12 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For GPR12 (Source: Uniprot.org, NCBI)
Gene Name
GPR12
Full Name
G-protein coupled receptor 12
Weight
36730 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
GPR12;G-protein coupled receptor 12 GPR12 GPCR12, GPCR21, PPP1R84 G protein-coupled receptor 12 G-protein coupled receptor 12|protein phosphatase 1, regulatory subunit 84
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPR12, check out the GPR12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPR12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GPR12/Gpcr12 Antibody (A10818)
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No publications found for A10818
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Customer Q&As
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3 Customer Q&As for Anti-GPR12/Gpcr12 Antibody
Question
Can you help my question with product A10818, anti-GPR12/Gpcr12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10818 anti-GPR12/Gpcr12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-30
Question
We are currently using anti-GPR12/Gpcr12 antibody A10818 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
The anti-GPR12/Gpcr12 antibody (A10818) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-09
Question
Do you have a BSA free version of anti-GPR12/Gpcr12 antibody A10818 available?
Verified Customer
Verified customer
Asked: 2018-03-30
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR12/Gpcr12 antibody A10818 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-30
