Anti-GPR12/Gpcr12 Antibody

GPR12 antibody

Boster Bio Anti-GPR12/Gpcr12 Antibody (Catalog# A10818). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Product Info Summary

SKU: A10818
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, ICC, WB

Product Name

Anti-GPR12/Gpcr12 Antibody

View all GPR12 Antibodies

SKU/Catalog Number

A10818

Size

100ul

Form

Liquid

Description

Boster Bio Anti-GPR12/Gpcr12 Antibody (Catalog# A10818). Tested in WB, IF, ICC, ELISA applications. This antibody reacts with Human, Rat, Mouse.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR12/Gpcr12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10818)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human GPR12. Immunogen sequence location: 18264

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10818 is reactive to GPR12 in Human, Mouse, Rat

Applications

A10818 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

36730 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:5000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For GPR12 (Source: Uniprot.org, NCBI)

Gene Name

GPR12

Full Name

G-protein coupled receptor 12

Weight

36730 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

FLJ18149; FLJ97704; G protein-coupled receptor 12; GPCR12; GPCR21; GPR12; G-protein coupled receptor 12; MGC138349; MGC138351 GPR12 GPCR12, GPCR21, PPP1R84 G protein-coupled receptor 12 G-protein coupled receptor 12|protein phosphatase 1, regulatory subunit 84

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPR12, check out the GPR12 Infographic

GPR12 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPR12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-GPR12/Gpcr12 Antibody

Question

Can you help my question with product A10818, anti-GPR12/Gpcr12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10818 anti-GPR12/Gpcr12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-30

Question

We are currently using anti-GPR12/Gpcr12 antibody A10818 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2018-07-09

Answer

The anti-GPR12/Gpcr12 antibody (A10818) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-07-09

Question

Do you have a BSA free version of anti-GPR12/Gpcr12 antibody A10818 available?

Verified Customer

Verified customer

Asked: 2018-03-30

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR12/Gpcr12 antibody A10818 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-30

Order DetailsPrice
A10818

100 μL

$335

Free Secondary Antibody

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A10818
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$335.00

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