Anti-GPR100 RXFP4 Antibody

RXFP4/GPCR142/GPR100 antibody

Boster Bio Anti-GPR100 RXFP4 Antibody catalog # A09371. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A09371
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-GPR100 RXFP4 Antibody

View all RXFP4/GPCR142/GPR100 Antibodies

SKU/Catalog Number

A09371

Size

100μl

Form

Liquid

Description

Boster Bio Anti-GPR100 RXFP4 Antibody catalog # A09371. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GPR100 RXFP4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09371)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the C-terminal region of human GPR100. at AA rangle: 300-380

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09371 is reactive to Rxfp4 in Human

Applications

A09371 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

41141 MW

Background of RXFP4/GPCR142/GPR100

APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Rxfp4 (Source: Uniprot.org, NCBI)

Gene Name

Rxfp4

Full Name

Relaxin-3 receptor 2

Weight

41141 MW

Superfamily

G-protein coupled receptor 1 family

Alternative Names

G protein-coupled receptor 100; GPCR142; GPR100; Relaxin-3 receptor-2; RLN3R2; RXFP4 relaxin/insulin-like family peptide receptor 4; RXFP4; RXFPR4; SALPR

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Rxfp4, check out the Rxfp4 Infographic

Rxfp4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Rxfp4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-GPR100 RXFP4 Antibody

Question

Would anti-GPR100 antibody A09371 work on canine WB with brain?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

Our lab technicians have not tested anti-GPR100 antibody A09371 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GPR100 antibody A09371 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-11

Question

We are currently using anti-GPR100 antibody A09371 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

The anti-GPR100 antibody (A09371) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-08

Question

Does A09371 anti-GPR100 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Evans

Verified customer

Asked: 2015-08-18

Answer

It shows on the product datasheet, A09371 anti-GPR100 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-08-18

Question

I have attached the WB image, lot number and protocol we used for pancreatic carcinoma using anti-GPR100 antibody A09371. Please let me know if you require anything else.

Z. Singh

Verified customer

Asked: 2015-05-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-05-28

Order DetailsPrice
A09371

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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