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Product Info Summary
| SKU: | A13524 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GOLGIN97 GOLGA1 Antibody
SKU/Catalog Number
A13524
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GOLGIN97 GOLGA1 Antibody (Catalog # A13524). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GOLGIN97 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-GOLGIN97 GOLGA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13524)
Host
Rabbit
Contents
GOLGIN97 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GOLGIN97 antibody was raised against a 17 amino acid peptide near the amino terminus of human GOLGIN97. The immunogen is located within amino acids 30 - 80 of GOLGIN97.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
GOLGIN97 antibody is human, mouse and rat reactive. At least two isoforms of GOLGIN97 are known to exist; this antibody will detect only the longer isoform. GOLGIN97 antibody is predicted to not cross-react with other GOLGIN family proteins.
Reactive Species
A13524 is reactive to GOLGA1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
88184 MW
Background of Golgin 97
GOLGIN97 was initially isolated as a Golgi complex autoantigen associated with Sjogren's syndrome, a chronic autoimmune disease in which the body's white blood cells destroy the exocrine glands, specifically the salivary and lacrimal glands (1,2). GOLGIN97 plays a role as a tethering molecule in endosome to the trans-Golgi network (TGN) retrograde traffic (3). FIP1/RCP binding to GOLGIN97 is required for tethering and fusion of recycling endosome retrograde transport vesicles to the TGN (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13524 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GOLGIN97 antibody can be used for detection of GOLGIN97 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GOLGIN97 in human stomach tissue lysate with GOLGIN97 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking petpide.
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Immunohistochemistry of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 5 μg/mL.
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Immunofluorescence of GOLGIN97 in rat stomach tissue with GOLGIN97 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For GOLGA1 (Source: Uniprot.org, NCBI)
Gene Name
GOLGA1
Full Name
Golgin subfamily A member 1
Weight
88184 MW
Alternative Names
Golgin subfamily A member 1, GOLGA1, GOLGIN A1, GOLGIN-97 GOLGA1 golgin-97 golgin A1 golgin subfamily A member 1|gap junction protein, alpha 4, 37kD|golgi auto, golgin subfamily a, 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOLGA1, check out the GOLGA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOLGA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GOLGIN97 GOLGA1 Antibody (A13524)
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