Product Info Summary
SKU: | M13524 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-Golgin 97 GOLGA1 Monoclonal Antibody
SKU/Catalog Number
M13524
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Golgin 97 GOLGA1 Monoclonal Antibody catalog # M13524. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Golgin 97 GOLGA1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13524)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACAD-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Golgin 97 The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogren's syndrome. The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M13524 is reactive to GOLGA1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
18 kDa
Calculated molecular weight
15404 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13524 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:30
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Golgin 97 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For GOLGA1 (Source: Uniprot.org, NCBI)
Gene Name
GOLGA1
Full Name
Golgin subfamily A member 1
Weight
15404 MW
Alternative Names
Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3/f;Histone H3/h;Histone H3/i;Histone H3/j;Histone H3/k;Histone H3/l;HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ; GOLGA1 golgin-97 golgin A1 golgin subfamily A member 1|gap junction protein, alpha 4, 37kD|golgi auto, golgin subfamily a, 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOLGA1, check out the GOLGA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOLGA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Golgin 97 GOLGA1 Monoclonal Antibody (M13524)
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No publications found for M13524
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Customer Q&As
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7 Customer Q&As for Anti-Golgin 97 GOLGA1 Monoclonal Antibody
Question
We are currently using anti-Golgin 97 Monoclonal antibody M13524 for human tissue, and we are well pleased with the IP results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-25
Answer
The anti-Golgin 97 Monoclonal antibody (M13524) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-25
Question
Will anti-Golgin 97 Monoclonal antibody M13524 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
According to the expression profile of liver, GOLGA1 is highly expressed in liver. So, it is likely that anti-Golgin 97 Monoclonal antibody M13524 will work for WB with liver.
Boster Scientific Support
Answered: 2019-10-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Golgin 97 Monoclonal antibody M13524. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-17
Question
I was wanting to use your anti-Golgin 97 Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
It shows on the product datasheet, M13524 anti-Golgin 97 Monoclonal antibody has been tested for IP, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-21
Question
See attached the WB image, lot number and protocol we used for liver using anti-Golgin 97 Monoclonal antibody M13524. Please let me know if you require anything else.
T. Mitchell
Verified customer
Asked: 2018-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-27
Question
I see that the anti-Golgin 97 Monoclonal antibody M13524 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-04
Question
Is a blocking peptide available for product anti-Golgin 97 Monoclonal antibody (M13524)?
K. Brown
Verified customer
Asked: 2014-05-07
Answer
We do provide the blocking peptide for product anti-Golgin 97 Monoclonal antibody (M13524). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-05-07