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Product Info Summary
| SKU: | A10740-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-GOLGA7 Antibody Picoband™
SKU/Catalog Number
A10740-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GOLGA7 Antibody Picoband™ catalog # A10740-1. Tested in ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GOLGA7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10740-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
E.coli-derived human GOLGA7 recombinant protein (Position: R2-R131). Human GOLGA7 shares 100% and 99.2% amino acid (aa) sequence identity with mouse and rat GOLGA7, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A10740-1 is reactive to GOLGA7 in Human
Applications
A10740-1 is guaranteed for ELISA, IHC Boster Guarantee
Observed Molecular Weight
75 kDa
Calculated molecular weight
52588 MW
Background of GOLGA7
Golgin A7 is a protein that in humans is encoded by the GOLGA7 gene. GOLGA7 (Golgin A7) is a Protein Coding gene. Among its related pathways are Innate Immune System. An important paralog of this gene is GOLGA7B.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 2-5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human rectum adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of GOLGA7 using anti-GOLGA7 antibody (A10740-1).
GOLGA7 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GOLGA7 Antibody (A10740-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For GOLGA7 (Source: Uniprot.org, NCBI)
Gene Name
GOLGA7
Full Name
Golgin subfamily A member 7
Weight
52588 MW
Superfamily
ERF4 family
Alternative Names
GCP16MGC21096; GOLGA3AP1; GOLGA7A; golgi autoantigen, golgin subfamily a, 7; Golgi complex-associated protein of 16 kDa; Golgi complex-associated protein of 16kDa; golgin A7; Golgin subfamily A member 7; HSPC041; MGC4876 GOLGA7 GCP16, GOLGA3AP1A, HSPC041, GOLGA7 golgin A7 golgin subfamily A member 7|Golgi complex-associated protein of 16kDa|golgi autoantigen, golgin subfamily a, 7|golgi complex-associated protein of 16 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GOLGA7, check out the GOLGA7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GOLGA7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GOLGA7 Antibody Picoband™ (A10740-1)
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