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Product Info Summary
| SKU: | M13077 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-GNG3 Antibody (C-Term)
SKU/Catalog Number
M13077
Size
50 µl
Description
Boster Bio Anti-GNG3 Antibody (C-Term) (Catalog # M13077). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-GNG3 Antibody (C-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13077)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This GNG3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from human GNG3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M13077 is reactive to GNG3 in Human, Mouse
Reconstitution
Calculated molecular weight
8305 Da
Background of G gamma3
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13077 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
FC: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-GNG3 Antibody (C-Term) at 1:2000 dilution
Lane 1: human fetal brain lysate
Lane 2: mouse brain lysate
Lane 3: human brain lysate
Lane 4: mouse cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 8 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-87 MG cells stained with M13077(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M13077, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For GNG3 (Source: Uniprot.org, NCBI)
Gene Name
GNG3
Full Name
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3
Weight
8305 Da
Superfamily
G protein gamma family
Alternative Names
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3 GNG3 G protein subunit gamma 3 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3|NBP gamma-3|guanine nucleotide binding protein (G protein), gamma 3|guanine nucleotide-binding protein gamma-3 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNG3, check out the GNG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNG3 Antibody (C-Term) (M13077)
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