Product Info Summary
SKU: | A13707 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-GNG11 Antibody Picoband™
SKU/Catalog Number
A13707
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GNG11 Antibody Picoband™ catalog # A13707. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GNG11 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13707)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GNG11 recombinant protein (Position: M1-S73).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A13707 is reactive to GNG11 in Human, Mouse, Rat
Applications
A13707 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
10 kDa
Calculated molecular weight
64099 MW
Background of GNG11
Guanine nucleotide-binding protein G (I)/G (S)/G (O) subunit gamma-11 is a protein that in humans is encoded by the GNG11 gene. This gene is a member of the guanine nucleotide-binding protein (G protein) gamma family and encodes a lipid-anchored, cell membrane protein. As a member of the heterotrimeric G protein complex, this protein plays a role in this transmembrane signaling system. This protein is also subject to carboxyl-terminal processing. Decreased expression of this gene is associated with splenic marginal zone lymphomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
![a13707 gng11 primary antibodies wb testing 1 a13707 gng11 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of GNG11 using anti-GNG11 antibody (A13707).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse kidney tissue lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: human A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNG11 antigen affinity purified polyclonal antibody (Catalog # A13707) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNG11 at approximately 10KD. The expected band size for GNG11 is at 10KD.
![a13707 gng11 primary antibodies ihc testing 2 a13707 gng11 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-2.jpg)
Click image to see more details
Figure 2. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 3 a13707 gng11 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 4 a13707 gng11 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 5 a13707 gng11 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 6 a13707 gng11 primary antibodies ihc testing 6](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-6.jpg)
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Figure 6. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 7 a13707 gng11 primary antibodies ihc testing 7](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-7.jpg)
Click image to see more details
Figure 7. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human skin cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 8 a13707 gng11 primary antibodies ihc testing 8](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-8.jpg)
Click image to see more details
Figure 8. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human melanoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies ihc testing 9 a13707 gng11 primary antibodies ihc testing 9](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-ihc-testing-9.jpg)
Click image to see more details
Figure 9. IHC analysis of GNG11 using anti-GNG11 antibody (A13707).
GNG11 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG11 Antibody (A13707) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a13707 gng11 primary antibodies fcm testing 10 a13707 gng11 primary antibodies fcm testing 10](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-fcm-testing-10.png)
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Figure 10. Flow Cytometry analysis of RH35 cells using anti-GNG11 antibody (A13707).
Overlay histogram showing RH35 cells stained with A13707 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG11 Antibody (A13707, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a13707 gng11 primary antibodies fcm testing 11 a13707 gng11 primary antibodies fcm testing 11](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-fcm-testing-11.png)
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Figure 11. Flow Cytometry analysis of U87 cells using anti-GNG11 antibody (A13707).
Overlay histogram showing U87 cells stained with A13707 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG11 Antibody (A13707, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
![a13707 gng11 primary antibodies fcm testing 12 a13707 gng11 primary antibodies fcm testing 12](https://www.bosterbio.com/media/catalog/product/a/1/a13707-gng11-primary-antibodies-fcm-testing-12.png)
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Figure 12. Flow Cytometry analysis of HEPA1-6 cells using anti-GNG11 antibody (A13707).
Overlay histogram showing HEPA1-6 cells stained with A13707 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG11 Antibody (A13707, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GNG11 (Source: Uniprot.org, NCBI)
Gene Name
GNG11
Full Name
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11
Weight
64099 MW
Superfamily
G protein gamma family
Alternative Names
GNGT11G protein gamma-11 subunit; guanine nucleotide binding protein (G protein), gamma 11; guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-11 subunit; guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11 GNG11 GNGT11 G protein subunit gamma 11 guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-11|G protein gamma-11 subunit|guanine nucleotide binding protein (G protein), gamma 11|guanine nucleotide-binding protein G(I)/G(S)/G(O) gamma-11 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GNG11, check out the GNG11 Infographic
![GNG11 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GNG11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GNG11 Antibody Picoband™ (A13707)
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