Product Info Summary
SKU: | A12403 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IHC |
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Product info
Product Name
Anti-GMF- gamma Antibody
SKU/Catalog Number
A12403
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GMF- gamma Antibody catalog # A12403. Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GMF- gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12403)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 1-50.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12403 is reactive to GMFG in Human
Applications
A12403 is guaranteed for IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
16801 MW
Background of GMFG
COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERα) and repress ERα-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogenesis (Zhong H et al., 2004)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:200.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For GMFG (Source: Uniprot.org, NCBI)
Gene Name
GMFG
Full Name
Glia maturation factor gamma
Weight
16801 MW
Superfamily
actin-binding proteins ADF family
Alternative Names
glia maturation factor gamma; glia maturation factor, gamma; GMF-GAMMA; MGC126867 GMFG GMF-GAMMA glia maturation factor gamma glia maturation factor gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GMFG, check out the GMFG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GMFG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GMF- gamma Antibody (A12403)
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No publications found for A12403
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Customer Q&As
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5 Customer Q&As for Anti-GMF- gamma Antibody
Question
Would anti-GMF- gamma antibody A12403 work on feline IHC with brain?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
Our lab technicians have not validated anti-GMF- gamma antibody A12403 on feline. You can run a BLAST between feline and the immunogen sequence of anti-GMF- gamma antibody A12403 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-07
Question
Is a blocking peptide available for product anti-GMF- gamma antibody (A12403)?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
We do provide the blocking peptide for product anti-GMF- gamma antibody (A12403). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-27
Question
We are currently using anti-GMF- gamma antibody A12403 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
The anti-GMF- gamma antibody (A12403) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-04
Question
Is this A12403 anti-GMF- gamma antibody reactive to the isotypes of GMFG?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
The immunogen of A12403 anti-GMF- gamma antibody is Synthetic peptide from human protein at AA range: 1-50. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-26
Question
Will A12403 anti-GMF- gamma antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Li
Verified customer
Asked: 2017-01-05
Answer
You can see on the product datasheet, A12403 anti-GMF- gamma antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-05