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Product Info Summary
| SKU: | A09506-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-GLYAT Antibody Picoband®
SKU/Catalog Number
A09506-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GLYAT Antibody Picoband® catalog # A09506-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GLYAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09506-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GLYAT recombinant protein (Position: K44-L296). Human GLYAT shares 70.8% and 73.5% amino acid (aa) sequence identity with mouse and rat GLYAT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09506-1 is reactive to GLYAT in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
34 kDa
Calculated molecular weight
34 kDa
Background of GLYAT
Glycine-N-acyltransferase, also known as GLYAT, is an enzyme which in humans is encoded by the GLYAT gene. The glycine-N-acyltransferase protein conjugates glycine with acyl-CoA substrates in the mitochondria. The protein is thought to be important in the detoxification of endogenous and xenobiotic acyl-CoA's. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09506-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg /1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human hepatocellular carcinoma tumor tissue (HCCT), rat kidney tissue, rat liver tissue, mouse kidney tissue, mouse liver tissue
FCM: RT4 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GLYAT using anti-GLYAT antibody (A09506-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat liver tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLYAT antigen affinity purified polyclonal antibody (Catalog # A09506-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLYAT at approximately 34 kDa. The expected band size for GLYAT is at 34 kDa.
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Figure 2. Flow Cytometry analysis of RT4 cells using anti-GLYAT antibody (A09506-1).
Overlay histogram showing RT4 cells stained with A09506-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLYAT Antibody (A09506-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For GLYAT (Source: Uniprot.org, NCBI)
Gene Name
GLYAT
Full Name
Glycine N-acyltransferase
Weight
34 kDa
Superfamily
glycine N-acyltransferase family
Alternative Names
ACGNATAAc; Acyl-CoA:glycine N-acyltransferase; Aralkyl acyl-CoA N-acyltransferase; Aralkyl acyl-CoA:amino acid N-acyltransferase; aralkyl-CoA N-acyltransferase; CATEC 2.3.1.13; GATHRP-1(CLP); glycine N-acyltransferase; glycine-N-acyltransferase GLYAT ACGNAT, CAT, GAT glycine-N-acyltransferase glycine N-acyltransferase|AAc|HRP-1(CLP)|acyl-CoA:glycine N-acyltransferase|aralkyl acyl-CoA N-acyltransferase|aralkyl acyl-CoA:amino acid N-acyltransferase|aralkyl-CoA N-acyltransferase|benzoyl-coenzyme A:glycine N-acyltransferase|epididymis secretory sperm binding protein|glycine N-benzoyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GLYAT, check out the GLYAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GLYAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GLYAT Antibody Picoband® (A09506-1)
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