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Product Info Summary
| SKU: | M02905 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-GluR2 Monoclonal Antibody
SKU/Catalog Number
M02905
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-GluR2 Monoclonal Antibody catalog # M02905. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GluR2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02905)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACIB-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human GluR2 Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02905 is reactive to GRIA2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
136 kDa
Calculated molecular weight
35197 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02905 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of GluR2 expression in (1) Human fetal brain lysate; (2) Mouse brain lysate.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For GRIA2 (Source: Uniprot.org, NCBI)
Gene Name
GRIA2
Full Name
Glutamate receptor 2
Weight
35197 MW
Superfamily
glutamate-gated ion channel (TC 1.A.10.1) family
Alternative Names
RNA-binding protein Musashi homolog 2;Musashi-2;MSI2; GRIA2 GLUR2, GLURB, GluA2, GluR-K2, HBGR2, NEDLIB, gluR-2, gluR-B glutamate ionotropic receptor AMPA type subunit 2 glutamate receptor 2|AMPA-selective glutamate receptor 2|glutamate receptor, ionotropic, AMPA 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRIA2, check out the GRIA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRIA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GluR2 Monoclonal Antibody (M02905)
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Customer Q&As
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4 Customer Q&As for Anti-GluR2 Monoclonal Antibody
Question
Is a blocking peptide available for product anti-GluR2 Monoclonal antibody (M02905)?
S. Collins
Verified customer
Asked: 2020-04-09
Answer
We do provide the blocking peptide for product anti-GluR2 Monoclonal antibody (M02905). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-09
Question
Does anti-GluR2 Monoclonal antibody M02905 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Our lab technicians have not validated anti-GluR2 Monoclonal antibody M02905 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GluR2 Monoclonal antibody M02905 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-26
Question
What sample target on Western Blot application was tested with M02905?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of GluR2 expression in (1) Human fetal brain lysate; (2) Mouse brain lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
My question regarding product M02905, anti-GluR2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-22
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02905 anti-GluR2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-22
