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Product Info Summary
| SKU: | A00678-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Glucagon GCG Antibody
SKU/Catalog Number
A00678-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Glucagon GCG Antibody catalog # A00678-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucagon GCG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00678-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human PLC gamma1 around the phosphorylation site of Y783.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00678-1 is reactive to GCG in Human, Mouse, Rat
Applications
A00678-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
20909 MW
Background of GCG
Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.
Denis Tvorogov, J. Cell Sci., Feb 2005; 118: 601 - 610.
Brenda J. Irvin, Mol. Cell. Biol., Dec 2000; 20: 9149 - 9161.
Benoit Poulin, J. Biol. Chem., Feb 2000; 275: 6411.
Ichiro Nakamura, J. Cell Biol., Jan 2001; 152: 361.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
IHC: 1:50-1:200
Validation Images & Assay Conditions
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Figure 1. Immunohistochemistry validation of GCG using Anti-Glucagon GCG Antibody (A00678-1).
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.For more protocol information of IHC
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Figure 2. Immunohistochemistry validation of GCG using Anti-Glucagon GCG Antibody (A00678-1).
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For GCG (Source: Uniprot.org, NCBI)
Gene Name
GCG
Full Name
Glucagon
Weight
20909 MW
Superfamily
glucagon family
Alternative Names
GCG; glicentin-related polypeptide; GLP1; GLP-1; Glucagon; glucagon-like peptide 1; glucagon-like peptide 2; GRPP GCG GLP-1, GLP1, GLP2, GRPP glucagon pro-glucagon|glicentin-related polypeptide|glucagon-like peptide 1|glucagon-like peptide 2|preproglucagon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCG, check out the GCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Glucagon GCG Antibody (A00678-1)
Hello CJ!
A00678-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huo K,Li X,Hu W,Song X,Zhang D,Zhang X,Chen X,Yuan J,Zuo J,Wang X.RFRP-3, the Mammalian Ortholog of GnIH, Is a Novel Modulator Involved in Food Intake and Glucose Homeostasis.Front Endocrinol (Lausanne). 2020 Apr 9;11:194.doi:10.3389/fendo.2020.00194.PMID
Species: Rat
A00678-1 usage in article: APP:IHC, SAMPLE:PANCREATIC TISSUE, DILUTION:1:100
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