Anti-GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Monoclonal Antibody

Product Name

Anti-GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Monoclonal Antibody

View all Golgi Glycoprotein 1/GLG1 Antibodies

SKU/Catalog Number

M07510

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Monoclonal Antibody (Catalog # M07510). Tested in IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07510)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: GLG1/970

Isotype

IgG1, kappa

Immunogen

Golgi fraction from human liver cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M07510 is reactive to GLG1 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

134552 MW

Background of Golgi Glycoprotein 1/GLG1

This monoclonal antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This monoclonal antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M07510 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For GLG1 (Source: Uniprot.org, NCBI)

Alternative Names

Golgi apparatus protein 1;CFR-1;Cysteine-rich fibroblast growth factor receptor;E-selectin ligand 1;ESL-1;Golgi sialoglycoprotein MG-160;GLG1;CFR1, ESL1, MG160; GLG1 CFR-1, ESL-1, MG-160, MG160 golgi glycoprotein 1 Golgi apparatus protein 1|E-selectin ligand 1|cysteine-rich fibroblast growth factor receptor|golgi sialoglycoprotein MG-160

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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