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Product Info Summary
| SKU: | PB9596 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-GJA3 Antibody Picoband®
SKU/Catalog Number
PB9596
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GJA3 Antibody Picoband® catalog # PB9596. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GJA3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9596)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GJA3, different from the related mouse and rat sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9596 is reactive to GJA3 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
47410 MW
Background of GJA3
Gap junction alpha-3 protein, also known as Connexin-46, is a protein that in humans is encoded by the GJA3 gene. This gene is mapped to 13q12.11. The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9596 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Cardiac Muscle Tissue, Rat Kidney Tissue, Human Placenta Tissue, NIH3T3 Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GJA3 using anti-GJA3 antibody (PB9596).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug,
Lane 2: Rat Kidney Tissue Lysate at 50ug,
Lane 3: Human Placenta Tissue Lysate at 50ug,
Lane 4: NIH3T3 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA3 antigen affinity purified polyclonal antibody (Catalog # PB9596) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA3 at approximately 55 kDa. The expected band size for GJA3 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GJA3 (Source: Uniprot.org, NCBI)
Gene Name
GJA3
Full Name
Gap junction alpha-3 protein
Weight
47410 MW
Superfamily
connexin family
Alternative Names
Gap junction alpha-3 protein;Connexin-46;Cx46;GJA3; GJA3 CTRCT14, CX46, CZP3 gap junction protein alpha 3 gap junction alpha-3 protein|connexin-46|gap junction alpha 3|gap junction protein, alpha 3, 46kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJA3, check out the GJA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GJA3 Antibody Picoband® (PB9596)
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Customer Q&As
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6 Customer Q&As for Anti-GJA3 Antibody Picoband®
Question
I see that the anti-GJA3 antibody PB9596 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lens fibers using anti-GJA3 antibody PB9596. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Will anti-GJA3 antibody PB9596 work on primate WB with lens fibers?
C. Jones
Verified customer
Asked: 2019-11-21
Answer
Our lab technicians have not validated anti-GJA3 antibody PB9596 on primate. You can run a BLAST between primate and the immunogen sequence of anti-GJA3 antibody PB9596 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate lens fibers in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-GJA3 antibody PB9596 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
The anti-GJA3 antibody (PB9596) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-GJA3 antibody (PB9596)?
Verified Customer
Verified customer
Asked: 2018-11-30
Answer
We do provide the blocking peptide for product anti-GJA3 antibody (PB9596). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-11-30
Question
Do you have a BSA free version of anti-GJA3 antibody PB9596 available?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GJA3 antibody PB9596 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-12
