Anti-GGA2 Antibody Picoband™

Product Name

Anti-GGA2 Antibody Picoband™

View all GGA2 Antibodies

SKU/Catalog Number

A04016-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GGA2 Antibody Picoband™ catalog # A04016-2. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GGA2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04016-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human GGA2 recombinant protein (Position: L26-A608). Human GGA2 shares 85.4% amino acid (aa) sequence identity with mouse GGA2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04016-2 is reactive to GGA2 in Human

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Observed Molecular Weight

70 kDa

Calculated molecular weight

Background of GGA2

ADP-ribosylation factor-binding protein GGA2 is a protein that in humans is encoded by the GGA2 gene. ADP-ribosylation factor-binding protein GGA2 is a protein that in humans is encoded by the GGA2 gene. This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. This family member may play a significant role in cargo molecules regulation and clathrin-coated vesicle assembly.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04016-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Positive Control

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GGA2 using anti-GGA2 antibody (A04016-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GGA2 antigen affinity purified polyclonal antibody (Catalog # A04016-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GGA2 at approximately 70 kDa. The expected band size for GGA2 is at 67 kDa.

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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-GGA2 antibody (A04016-2).
Overlay histogram showing HepG2 cells stained with A04016-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GGA2 Antibody (A04016-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For GGA2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ20966; Gamma-adaptin-related protein 2; golgi associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; Golgi-localized, gamma ear-containing, ARF-binding protein 2; KIAA1080ADP-ribosylation factor-binding protein GGA2; VEAR; VHS domain and ear domain of gamma-adaptin; VHS domain and ear domain-containing protein GGA2 VEAR golgi associated, gamma adaptin ear containing, ARF binding protein 2 ADP-ribosylation factor-binding protein GGA2|VHS domain and ear domain of gamma-adaptin|VHS domain and ear domain-containing protein|gamma-adaptin-related protein 2|golgi-localized, gamma ear-containing, ARF-binding protein 2

For more info on GGA2, check out the GGA2 Infographic

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In this infographic, you will see the following information for GGA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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