Product Info Summary
SKU: | A03067 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-GBP1 (E99) Antibody
SKU/Catalog Number
A03067
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GBP1 (E99) Antibody catalog # A03067. Tested in WB,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GBP1 (E99) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03067)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03067 is reactive to GBP1 in Human, Mouse, Rat
Applications
A03067 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
67931 MW
Background of GBP1
RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IF: 1:50-1:200
Validation Images & Assay Conditions
![a03067 anti gbp1 e99 antibody wb testing 1 a03067 anti gbp1 e99 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03067-anti-gbp1-e99-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-GBP1 (E99) Antibody A03067
Western blot (WB) analysis of GBP1 (E99) pAb at 1:1000 dilutionLane1:HEK293T whole cell lysate(10ug)
Lane2:HCT116 whole cell lysate(10ug)
Lane3:The Kidney tissue lysate of Rat(20ug)
Lane4:The Kidney tissue lysate of Mouse(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For GBP1 (Source: Uniprot.org, NCBI)
Gene Name
GBP1
Full Name
Guanylate-binding protein 1
Weight
67931 MW
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
GBP-1; GTP-binding protein 1; Guanine nucleotide-binding protein 1; guanylate binding protein 1, interferon-inducible; guanylate binding protein 1, interferon-inducible, 67kDa; huGBP-1; interferon-induced guanylate-binding protein 1 GBP1 guanylate binding protein 1 guanylate-binding protein 1|GBP-1|GTP-binding protein 1|guanine nucleotide-binding protein 1|guanylate binding protein 1, interferon-inducible, 67kDa|huGBP-1|interferon-induced guanylate-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GBP1, check out the GBP1 Infographic
![GBP1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GBP1 (E99) Antibody (A03067)
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