Product Info Summary
SKU: | M06121 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Pig |
Host: | Mouse |
Application: | Flow Cytometry, ICC, WB |
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Product info
Product Name
Anti-GAPDHS Purified Monoclonal Antibody
SKU/Catalog Number
M06121
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GAPDHS Purified Monoclonal Antibody (Catalog# M06121). Tested in WB, ICC, Flow Cytometry application(s). This antibody reacts with Pig, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-GAPDHS Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06121)
Host
Mouse
Contents
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
Hs-8
Isotype
Mouse IgM
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization. The antibody Hs-8 reacts with GAPDHS, the sperm-specific glyceraldehyde phosphate dehydrogenase, which is an intra-acrosomal protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Cross-reactivity with CLIC5b was not determined.
Reactive Species
M06121 is reactive to GAPDHS in Human, Pig
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
44501 MW
Background of GAPDH-2
GAPDHS (the sperm-specific glyceraldehyde phosphate dehydrogenase, also known as GAPD2, GAPDS, HSD-35, or GAPDH-2, is a glycolytic enzyme that plays an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. It can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06121 is guaranteed for Flow Cytometry, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry: 10 μg/ml; membrane permeabilization (acetone) is essential.
Flow cytometry: 3-12 µg/ml. Intraacrosomal staining.
Validation Images & Assay Conditions
There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.
Protein Target Info & Infographic
Gene/Protein Information For GAPDHS (Source: Uniprot.org, NCBI)
Gene Name
GAPDHS
Full Name
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific
Weight
44501 MW
Superfamily
glyceraldehyde-3-phosphate dehydrogenase family
Alternative Names
SGAPDH, HSD-35 Gapdhs|Ga, Gap, Gapd-s, Gapds|glyceraldehyde-3-phosphate dehydrogenase, spermatogenic|glyceraldehyde-3-phosphate dehydrogenase, testis-specific|GAPDH-2|spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2|spermatogenic glyceraldehyde-3-phosphate dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAPDHS, check out the GAPDHS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAPDHS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAPDHS Purified Monoclonal Antibody (M06121)
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