Click image to see more details
Product Info Summary
| SKU: | M01868-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Chicken, Equine, Human, Mouse, Pig, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GAP43 Antibody
SKU/Catalog Number
M01868-3
Size
100ul
Description
Boster Bio Anti-GAP43 Antibody catalog # M01868-3. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Chicken, Equine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAP43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01868-3)
Host
Rabbit
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Polyclonal
Immunogen
C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01868-3 is reactive to GAP43 in Bovine, Chicken, Equine, Human, Mouse, Pig, Rat
Reconstitution
Calculated molecular weight
24803 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01868-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blots: 1:10,000 IF/ICC and IHC: 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different tissue and cell lysates using rabbit pAb to GAP43, M01868-3, dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] SH-SY5Y cells, [7] C6 cells. Single band at 43 kDa mark corresponds to GAP43 protein. The GAP43 protein is detected only in the lysates of neuronal origin. C6 cells are a rat glioma cell line and do not express GAP43 protein.
Click image to see more details
Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat stained with rabbit pAb to GAP43, M01868-3 , dilution 1:2,000 in green, and costained with mouse mAb to vimentin, dilution 1:2,000, in red. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of neuronal cells, while vimentin antibody stains intermediate filaments in fibroblasts and other non-neuronal cells.
Protein Target Info & Infographic
Gene/Protein Information For GAP43 (Source: Uniprot.org, NCBI)
Gene Name
GAP43
Full Name
Neuromodulin
Weight
24803 MW
Superfamily
neuromodulin family
Alternative Names
Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Neural phosphoprotein B-50;pp46;GAP43; GAP43 B-50, GAP-43, PP46 growth associated protein 43 neuromodulin|axonal membrane protein GAP-43|calmodulin-binding protein P-57|nerve growth-related peptide GAP43|neural phosphoprotein B-50|neuron growth-associated protein 43|protein F1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GAP43, check out the GAP43 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GAP43: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GAP43 Antibody (M01868-3)
Hello CJ!
M01868-3 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Z, Liu H, Zeng ZH. Biomed Res Int. 2018 Mar 7;2018:8697913. doi: 10.1155/2018/8697913. eCollection 2018. Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Incre...
Liu H, Lv P, Zhu Y, Wu H, Zhang K, Xu F, Zheng L, Zhao J. Sci Rep. 2017 Jan 5;7:39869. doi: 10.1038/srep39869. Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GAP43 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GAP43 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-GAP43 Antibody
Question
We have been able to see staining in bovine brain. Are there any suggestions? Is anti-GAP43 antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
From what I have seen in literature brain does express GAP43. From what I have seen in Uniprot.org, GAP43 is expressed in anterior cingulate cortex, brain cortex subthalamic nucleus, brain, among other tissues. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-21
Question
I was wanting to use your anti-GAP43 antibody for IF for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
As indicated on the product datasheet, M01868-3 anti-GAP43 antibody has been validated for IF, IHC, WB on bovine, chicken, equine, human, mouse, pig, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
Is this M01868-3 anti-GAP43 antibody reactive to the isotypes of GAP43?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
The immunogen of M01868-3 anti-GAP43 antibody is C-terminal peptide of rodent GAP43, KEDPEADQEHA coupled to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-26
Question
My colleagues were content with the WB result of your anti-GAP43 antibody. However we have seen positive staining in anterior cingulate cortex cell membrane using this antibody. Is that expected? Could you tell me where is GAP43 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
From what I have seen in literature, anterior cingulate cortex does express GAP43. Generally GAP43 expresses in cell membrane. Regarding which tissues have GAP43 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Brain cortex, and Subthalamic nucleus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GAP43 antibody M01868-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-14
Question
Does M01868-3 anti-GAP43 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
As indicated on the product datasheet, M01868-3 anti-GAP43 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-05
Question
Would anti-GAP43 antibody M01868-3 work for IF with brain?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to the expression profile of brain, GAP43 is highly expressed in brain. So, it is likely that anti-GAP43 antibody M01868-3 will work for IF with brain.
Boster Scientific Support
Answered: 2019-10-15
Question
I am looking for using your anti-GAP43 antibody for l1cam interactions studies. Has this antibody been tested with western blotting on c6 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Thanks for your inquiry. This M01868-3 anti-GAP43 antibody is validated on mouse brain, c6 cells, neuronal cells. It is guaranteed to work for IF, IHC, WB in bovine, chicken, equine, human, mouse, pig, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-11
Question
We are currently using anti-GAP43 antibody M01868-3 for pig tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says bovine, chicken, equine, human, mouse, pig, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The anti-GAP43 antibody (M01868-3) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-14
Question
Our lab used your anti-GAP43 antibody for IHC on brain in a previous project. I am using bovine, and We want to use the antibody for WB next. Our lab want to know about examining brain as well as brain cortex subthalamic nucleus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
I looked at the website and datasheets of our anti-GAP43 antibody and it appears that M01868-3 has been validated on bovine in both IHC and WB. Thus M01868-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in bovine even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-16
Question
Is a blocking peptide available for product anti-GAP43 antibody (M01868-3)?
Verified Customer
Verified customer
Asked: 2018-01-03
Answer
We do provide the blocking peptide for product anti-GAP43 antibody (M01868-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-03
Question
My question regarding product M01868-3, anti-GAP43 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
B. Walker
Verified customer
Asked: 2017-09-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01868-3 anti-GAP43 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-26
Question
Here is the WB image, lot number and protocol we used for brain using anti-GAP43 antibody M01868-3. Please let me know if you require anything else.
R. Walker
Verified customer
Asked: 2015-04-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-13
Question
My lab would like to test anti-GAP43 antibody M01868-3 on rat brain for research purposes, then I may be interested in using anti-GAP43 antibody M01868-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Jones
Verified customer
Asked: 2015-03-16
Answer
The products we sell, including anti-GAP43 antibody M01868-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-03-16
Question
I see that the anti-GAP43 antibody M01868-3 works with IF, what is the protocol used to produce the result images on the product page?
H. Moore
Verified customer
Asked: 2013-10-02
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-10-02
Question
Do you have a BSA free version of anti-GAP43 antibody M01868-3 available?
G. Miller
Verified customer
Asked: 2013-05-10
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GAP43 antibody M01868-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-05-10
