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Product Info Summary
| SKU: | A03523-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-gamma Synuclein/SNCG Antibody Picoband®
View all gamma-Synuclein Antibodies
SKU/Catalog Number
A03523-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-gamma Synuclein/SNCG Antibody Picoband® catalog # A03523-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-gamma Synuclein/SNCG Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03523-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human gamma Synuclein/SNCG, which shares 53.8% amino acid (aa) sequence identity with rat SNCG.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03523-4 is reactive to SNCG in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
17 kDa
Calculated molecular weight
177298 MW
Background of gamma-Synuclein
Gamma-synuclein is a protein that in humans is encoded by the SNCG gene. This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03523-4 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human RT4 whole cell, human Hacat whole cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody (A03523-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody (Catalog # A03523-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.
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Figure 2. Flow Cytometry analysis of Hela cells using anti-gamma Synuclein/SNCG antibody (A03523-4).
Overlay histogram showing Hela cells stained with A03523-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-gamma Synuclein/SNCG Antibody (A03523-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SNCG (Source: Uniprot.org, NCBI)
Gene Name
SNCG
Full Name
Gamma-synuclein
Weight
177298 MW
Superfamily
synuclein family
Alternative Names
Laminin subunit gamma-1;Laminin B2 chain;Laminin-1 subunit gamma;Laminin-10 subunit gamma;Laminin-11 subunit gamma;Laminin-2 subunit gamma;Laminin-3 subunit gamma;Laminin-4 subunit gamma;Laminin-6 subunit gamma;Laminin-7 subunit gamma;Laminin-8 subunit gamma;Laminin-9 subunit gamma;S-laminin subunit gamma;S-LAM gamma;Lamc1;Lamb-2, Lamc-1; SNCG BCSG1, SR synuclein gamma gamma-synuclein|-synuclein|E pound-synuclein|breast cancer-specific gene 1 protein|persyn|synoretin|synuclein, gamma (breast cancer-specific protein 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SNCG, check out the SNCG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SNCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-gamma Synuclein/SNCG Antibody Picoband® (A03523-4)
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