Anti-gamma Synuclein/SNCG Antibody Picoband™

Product Name

Anti-gamma Synuclein/SNCG Antibody Picoband™

View all gamma-Synuclein Antibodies

SKU/Catalog Number

A03523-4

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-gamma Synuclein/SNCG Antibody Picoband™ catalog # A03523-4. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-gamma Synuclein/SNCG Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03523-4)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human gamma Synuclein/SNCG, which shares 53.8% amino acid (aa) sequence identity with rat SNCG.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A03523-4 is reactive to SNCG in Human

Applications

A03523-4 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

177298 MW

Background of gamma-Synuclein

Gamma-synuclein is a protein that in humans is encoded by the SNCG gene. This gene encodes a member of the synuclein family of proteins which are believed to be involved in the pathogenesis of neurodegenerative diseases. Mutations in this gene have also been associated with breast tumor development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of gamma Synuclein/SNCG using anti-gamma Synuclein/SNCG antibody (A03523-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-gamma Synuclein/SNCG antigen affinity purified polyclonal antibody (Catalog # A03523-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for gamma Synuclein/SNCG at approximately 17 kDa. The expected band size for gamma Synuclein/SNCG is at 13 kDa.

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Figure 2. Flow Cytometry analysis of Hela cells using anti-gamma Synuclein/SNCG antibody (A03523-4).
Overlay histogram showing Hela cells stained with A03523-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-gamma Synuclein/SNCG Antibody (A03523-4, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For SNCG (Source: Uniprot.org, NCBI)

Alternative Names

BCSG1; BCSG1PERSYN; Breast cancer-specific gene 1 protein; gamma-Synuclein; persyn; PRSN; SNCG; SRPRSN; Synoretin; synuclein, gamma (breast cancer-specific protein 1); Synuclein-gamma SNCG BCSG1, SR synuclein gamma gamma-synuclein|-synuclein|E pound-synuclein|breast cancer-specific gene 1 protein|persyn|synoretin|synuclein, gamma (breast cancer-specific protein 1)

For more info on SNCG, check out the SNCG Infographic

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In this infographic, you will see the following information for SNCG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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