Anti-GALNT2 Antibody Picoband™

Product Name

Anti-GALNT2 Antibody Picoband™

View all GALNT2 Antibodies

SKU/Catalog Number

A04458-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-GALNT2 Antibody Picoband™ catalog # A04458-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-GALNT2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04458-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Immunogen

E.coli-derived human GALNT2 recombinant protein (Position: H442-Q571). Human GALNT2 shares 96.9% amino acid (aa) sequence identity with mouse GALNT2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04458-1 is reactive to GALNT2 in Human, Rat

Applications

A04458-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

Background of GALNT2

Polypeptide N-acetylgalactosaminyltransferase 2 is an enzyme that in humans is encoded by the GALNT2 gene. This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of GALNT2 using anti-GALNT2 antibody (A04458-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT2 antigen affinity purified polyclonal antibody (Catalog # A04458-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT2 at approximately 65 kDa. The expected band size for GALNT2 is at 65 kDa.

Gene/Protein Information For GALNT2 (Source: Uniprot.org, NCBI)

Alternative Names

GalNAc-T2; GalNAc-T2EC 2.4.1.41; GALNT2; Polypeptide GalNac Transferase 2; polypeptide N-acetylgalactosaminyltransferase 2; pp-GaNTase 2; Protein-UDP acetylgalactosaminyltransferase 2; UDP-GalNAc transferase 2; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 2 (GalNAc-T2) GALNT2 CDG2T, GalNAc-T2 polypeptide N-acetylgalactosaminyltransferase 2 polypeptide N-acetylgalactosaminyltransferase 2|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)|polypeptide GalNAc transferase 2|pp-GaNTase 2

For more info on GALNT2, check out the GALNT2 Infographic

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In this infographic, you will see the following information for GALNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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