Click image to see more details
Product Info Summary
| SKU: | A04458-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GALNT2 Antibody Picoband®
SKU/Catalog Number
A04458-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-GALNT2 Antibody Picoband® catalog # A04458-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-GALNT2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04458-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GALNT2 recombinant protein (Position: H442-Q571). Human GALNT2 shares 96.9% amino acid (aa) sequence identity with mouse GALNT2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04458-1 is reactive to GALNT2 in Human, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
64.733kDa
Background of GALNT2
Polypeptide N-acetylgalactosaminyltransferase 2 is an enzyme that in humans is encoded by the GALNT2 gene. This gene encodes a member of the glycosyltransferase 2 protein family. Members of this family initiate mucin-type O-glycoslation of peptides in the Golgi apparatus. The encoded protein may be involved in O-linked glycosylation of the immunoglobulin A1 hinge region. This gene may influence triglyceride levels, and may be involved Type 2 diabetes, as well as several types of cancer. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04458-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A549 whole cell, human Hela whole cell, human MOLT-4 whole cell, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GALNT2 using anti-GALNT2 antibody (A04458-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MOLT-4 whole cell lysates,
Lane 4: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALNT2 antigen affinity purified polyclonal antibody (Catalog # A04458-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GALNT2 at approximately 65 kDa. The expected band size for GALNT2 is at 65 kDa.
Protein Target Info & Infographic
Gene/Protein Information For GALNT2 (Source: Uniprot.org, NCBI)
Gene Name
GALNT2
Full Name
Polypeptide N-acetylgalactosaminyltransferase 2
Weight
64.733kDa
Superfamily
glycosyltransferase 2 family
Alternative Names
GalNAc-T2; GalNAc-T2EC 2.4.1.41; GALNT2; Polypeptide GalNac Transferase 2; polypeptide N-acetylgalactosaminyltransferase 2; pp-GaNTase 2; Protein-UDP acetylgalactosaminyltransferase 2; UDP-GalNAc transferase 2; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2; UDP-N-acetyl-alpha-D-galactosamine:polypeptideN-acetylgalactosaminyltransferase 2 (GalNAc-T2) GALNT2 CDG2T, GalNAc-T2 polypeptide N-acetylgalactosaminyltransferase 2 polypeptide N-acetylgalactosaminyltransferase 2|UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2|UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)|polypeptide GalNAc transferase 2|pp-GaNTase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GALNT2, check out the GALNT2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GALNT2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GALNT2 Antibody Picoband® (A04458-1)
Hello CJ!
No publications found for A04458-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GALNT2 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-GALNT2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
