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Product Info Summary
| SKU: | PB10065 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-FSH Receptor/FSHR Antibody Picoband®
SKU/Catalog Number
PB10065
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-FSH Receptor/FSHR Antibody Picoband® catalog # PB10065. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-FSH Receptor/FSHR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10065)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FSH Receptor recombinant protein (Position: C18-N187). Human FSH Receptor shares 91.2% and 90% amino acid (aa) sequence identity with mouse and rat FSH Receptor, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10065 is reactive to FSHR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
78 kDa
Calculated molecular weight
78265 MW
Background of FSHR
The follicle-stimulating hormone receptor or FSH receptor (FSHR) is a transmembrane receptor that interacts with the follicle-stimulating hormone (FSH) and represents a G protein-coupled receptor (GPCR). This FSHR gene is mapped to chromosome 2p21 by fluorescence in situ hybridization. The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10065 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat testis tissue, rat ovary tissue, mouse testis tissue, mouse ovary tissue, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FSH Receptor using anti-FSH Receptor antibody (PB10065).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates，
Lane 2: rat ovary tissue lysates，
Lane 3: mouse testis tissue lysates，
Lane 4: mouse ovary tissue lysates,
Lane 5: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSH Receptor antigen affinity purified polyclonal antibody (Catalog # PB10065) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSH Receptor at approximately 78 kDa. The expected band size for FSH Receptor is at 78 kDa.
Protein Target Info & Infographic
Gene/Protein Information For FSHR (Source: Uniprot.org, NCBI)
Gene Name
FSHR
Full Name
Follicle-stimulating hormone receptor
Weight
78265 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Follicle-stimulating hormone receptor;FSH-R;Follitropin receptor;FSHR;LGR1; FSHR FSHR1O, LGR1, ODG1, FSHR follicle stimulating hormone receptor follicle-stimulating hormone receptor|FSH receptor|follitropin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FSHR, check out the FSHR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FSHR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FSH Receptor/FSHR Antibody Picoband® (PB10065)
Hello CJ!
PB10065 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Luo Q,Liu R,Wang L,Hou Y,Zhang H.The Effects of Inhibin B in the Chemotherapy Drug-Induced Premature Ovarian Insufficiency Mice and hPMSCs Treatment.Reprod Sci.2020 May;27(5):1148-1155.doi:10.1007/s43032-019-00128-y.Epub 2020 Jan 28.PMID:31993999.
Species: Human,Mouse
PB10065 usage in article: APP:WB, SAMPLE:OVARY TISSUE, DILUTION:1:150
Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro
Activation of phospholipase C-%u03B31 and translocation of phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase contribute to GL-V9-induced apoptosis in human %u2026
Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks
Evaluation of Follicular Synchronization Caused by Estrogen Administration and Its Reproductive Outcome
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Customer Q&As
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1 Customer Q&As for Anti-FSH Receptor/FSHR Antibody Picoband®
Question
We are currently using anti-FSH Receptor/FSHR antibody PB10065 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The anti-FSH Receptor/FSHR antibody (PB10065) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-07
