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Product Info Summary
| SKU: | PA1577 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
SKU/Catalog Number
PA1577
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody catalog # PA1577. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1577)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FOXP3, different from the related mouse sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1577 is reactive to FOXP3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
47244 MW
Background of Foxp3
FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1577 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Cell, SGC Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-FOXP3 antibody, PA1577, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: SGC Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For FOXP3 (Source: Uniprot.org, NCBI)
Gene Name
FOXP3
Full Name
Forkhead box protein P3
Weight
47244 MW
Alternative Names
Forkhead box protein P3;Scurfin;Forkhead box protein P3, C-terminally processed;Forkhead box protein P3 41 kDa form;FOXP3;IPEX;JM2; Foxp3|JM2, scu, scurfin, sf|forkhead box P3|forkhead box protein P3|scurfy
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXP3, check out the FOXP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Forkhead box protein P3 FOXP3 Antibody Picoband® (PA1577)
Hello CJ!
PA1577 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-35 improves Treg-mediated immune suppression in atherosclerotic mice
Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer
LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn?s disease
Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, Zhu F, Wang Q, Zhang L. Plos One. 2014 Jan 30;9(1):E87787. Doi: 10.1371/Journal.Pone.0087787. Ecollection 2014. The Modulation Of Endoplasmic Reticulum Stress By Chemical Chaperone Upregulates Immune Ne...
Hu M, Li K, Maskey N, Xu Z, Peng C, Wang B, Li Y, Yang G. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4685-94. Ecollection 2014. Decreased Intratumoral Foxp3 Tregs And Increased Dendritic Cell Density By Neoadjuvant Chemotherapy Associated With Favora...
Gai Xd, Song Y, Li C, Lei Ym, Yang B. Pathol Res Pract. 2013 Dec;209(12):774-8. Doi: 10.1016/J.Prp.2013.08.011. Epub 2013 Sep 6. Potential Role Of Plasmacytoid Dendritic Cells For Foxp3+ Regulatory T Cell Development In Human Colorectal Cancer And...
Gai Xd, Li C, Song Y, Lei Ym, Yang Bx. Biomed Rep. 2013 Mar;1(2):207-212. Epub 2012 Oct 29. In Situ Analysis Of Foxp3+ Regulatory T Cells And Myeloid Dendritic Cells In Human Colorectal Cancer Tissue And Tumor-Draining Lymph Node.
Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...
Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...
Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...
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Customer Q&As
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2 Customer Q&As for Anti-Forkhead box protein P3 FOXP3 Antibody Picoband®
Question
I am interested in using your anti-FOXP3 antibody for t cell homeostasis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We appreciate your inquiry. This PA1577 anti-FOXP3 antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-07
Question
We are currently using anti-FOXP3 antibody PA1577 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
The anti-FOXP3 antibody (PA1577) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-03
