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Product Info Summary
| SKU: | A00659-3 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-FOXM1 Antibody Picoband®
SKU/Catalog Number
A00659-3
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-FOXM1 Antibody Picoband® catalog # A00659-3. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-FOXM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00659-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human FOXM1 recombinant protein (Position: K48-Q763). Human FOXM1 shares 78.9% and 81.1% amino acid (aa) sequence identity with mouse and rat FOXM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00659-3 is reactive to FOXM1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
110 kDa
Calculated molecular weight
84.283kDa
Background of FOXM1
Forkhead box protein M1, also called FOXM1, is a protein that in humans is encoded by the FOXM1 gene. It is mapped to 12p13.33. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases and also in the control of cell proliferation. FOXM1 gene is now known as a human proto-oncogene. Abnormal upregulation of FOXM1 is involved in the oncogenesis of basal cell carcinoma, the most common human cancer worldwide. It was hypothesized that FOXM1 induces cancer initiation through stem/progenitor cell expansion. What's more, FOXM1 has been shown to modulate the epigenome. It was found that overexpression of FOXM1 "brain washes" normal cells to adopt cancer-like epigenome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00659-3 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Hela whole cell, human DLD-1 whole cell, human MCF-7 whole cell, human U2OS whole cell, rat thymus tissue, rat testis tissue, mouse thymus tissue, mouse testis tissue
ICC/IF: HELA cell
FCM: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of FOXM1 using anti-FOXM1 antibody (A00659-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human DLD-1 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat thymus tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse thymus tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXM1 antigen affinity purified polyclonal antibody (Catalog # A00659-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXM1 at approximately 110 kDa. The expected band size for FOXM1 is at 84 kDa.
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Figure 2. IF analysis of FOXM1 using anti-FOXM1 antibody (A00659-3) and anti-Beta Tubulin antibody (M01857-3).
FOXM1 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-FOXM1 Antibody (A00659-3) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) and DyLight®550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of Hela cells using anti-FOXM1 antibody (A00659-3).
Overlay histogram showing Hela cells stained with A00659-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXM1 Antibody (A00659-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FOXM1 (Source: Uniprot.org, NCBI)
Gene Name
FOXM1
Full Name
Forkhead box protein M1
Weight
84.283kDa
Alternative Names
FKHL16; FKHL16trident; forkhead box M1; forkhead box protein M1; Forkhead, drosophila, homolog-like 16; forkhead-like 16; Forkhead-related protein FKHL16; FoxM1; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11FOXM1B; HFH-11M-phase phosphoprotein 2; HNF-3; HNF-3/fork-head homolog 11; INS-1; MPHOSPH2; MPP-2; MPP2MPM-2 reactive phosphoprotein 2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells FOXM1 FKHL16A, FOXM1B, FOXM1C, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TRIDENT, FOXM1 forkhead box M1 forkhead box protein M1|Forkhead, drosophila, homolog-like 16|HNF-3/fork-head homolog 11|M-phase phosphoprotein 2|MPM-2 reactive phosphoprotein 2|forkhead box M1-D|forkhead-related protein FKHL16|hepatocyte nuclear factor 3 forkhead homolog 11|transcription factor Trident|winged-helix factor from INS-1 cells
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOXM1, check out the FOXM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOXM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-FOXM1 Antibody Picoband® (A00659-3)
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